1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily Stem Cell CD Proteins
  4. TNF Receptor Superfamily CD271/NGFR
  5. Nerve Growth Factor Receptor (NGFR)
  6. NGFR Protein, Rabbit (HEK293, His)

NGFR Protein, Rabbit (HEK293, His)

目录号: HY-P74699
COA 产品使用指南

Nerve Growth Factor Receptor (NGFR) 也称为 CD271、p75NTR、TNFRSF16,是神经营养蛋白家族的跨膜低亲和力受体。NGFR 没有内在的酪氨酸激酶活性。NGFR 可作为肿瘤抑制因子,负向调节细胞生长和增殖。NGF-NGFR 激活 NF-κB,从而抑制细胞凋亡,增加神经鞘瘤和乳腺癌细胞的存活率。NGFR 蛋白, Rabbit (HEK293, His) 是一种由 HEK293 细胞表达的重组蛋白,其 C 末端带有 His 标签,由 242 (M1-D242) 个氨基酸组成。

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描述

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells[3]. NGFR Protein, Human (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 250 amino acids (M1-N250) and is produced in HEK293 cells. NGFR Protein, Rabbit (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 242 amino acids (M1-D242) and is produced in HEK293 cells.

研究背景

神经生长因子受体(NGFR)不仅在神经组织中表达,也在非神经元正常细胞和癌细胞中表达,如血管周围细胞、牙髓细胞、淋巴滤泡树突状细胞、口腔粘膜和毛囊的基底上皮、前列腺基底细胞和肌上皮细胞[1]
人 NGFR 与小鼠 NGFR 蛋白具有 92.45% aa 序列同一性,与大鼠 NGFR 蛋白具有 92.42% aa 序列同一性。
神经生长因子受体(NGFR)是一种I型跨膜蛋白,是TNFR超家族的典型结构,缺乏内在的催化活性。NGFR 信号传导涉及 NF-kB(Rel/NF-kB 转录因子)的激活和转录因子 c-Jun 激酶 (JNK) 的磷酸化,以及神经酰胺产量的增加,导致基因转录或程序性细胞死亡[2]
NGFR 诱导 p53 依赖性细胞凋亡和细胞生长停滞以及抑制肿瘤生长[3]。低亲和力神经生长因子受体 (NGFR) p75NGFR 在没有神经生长因子 (NGF) 结合的情况下诱导细胞凋亡,但在与 NGF 结合时增强神经存活。NGFR 增强 β-淀粉样肽毒性[4]。NGFR 信号可诱导随后黑色素瘤抗原的下调,并最终抑制 CTL 激活[5]

生物活性

Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.163 µg/mL in the presence of 2 ng/mL of recombinant human beta-NGF, corresponding to a specific activity is 6.1350×10[3] units/mg.

种属

Rabbit

表达系统

HEK293

标签

C-His

蛋白编号

XP_008269543 (K22-D242)

基因 ID

100349095  [NCBI]

蛋白结构
N-term
NGFR Protein (K22-D242)
Accession # XP_008269543
His
C-term
中文名
重组兔 NGFR 蛋白 (HEK293, His)
同用名
NGFR; Gp80-LNGFR; p75 ICD; CD271; TNFRSF16
氨基酸序列

KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDTVCRCAYGYYQDETTGRCEAYRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECSRWADAECEAIPGRWTTRSPAPEGSDSMAPSTQVPEGPPEQELPASTAADVVTTVMGSSQPVATRGTAD

分子量

Approximately 43-50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NGFR Protein, Rabbit (HEK293, His)
目录号:
HY-P74699
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