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  4. NIP30 蛋白, Human (HEK293, His)

NIP30 蛋白, Human (HEK293, His)

目录号: HY-P701118
产品使用指南

NIP30 蛋白负向调节蛋白酶体蛋白分解代谢和蛋白结合活性。它位于细胞核中，并在包括大脑和睾丸在内的各种组织中表达。NIP30 蛋白, Human (HEK293, His) 是重组的 NIP30 蛋白，由 HEK293 表达，带有 C-His 标签。NIP30 蛋白, Human (HEK293, His) 全长 254 个氨基酸，糖基化后的分子量为 ~35-43 kDa。

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规格 价格 是否有货 数量
50 μg ￥8000 In-stock
100 μg   询价  

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描述

The NIP30 protein negatively regulates proteasomal protein catabolism and protein binding activity. It is located in the nucleus and is expressed in various tissues including the brain and testis. NIP30 Protein, Human (HEK293, His) is the recombinant human-derived NIP30 protein, expressed by HEK293, with C-His labeled tag. The total length of NIP30 Protein, Human (HEK293, His) is 254 a.a., with molecular weight (glycosylation form) of ~35-43 kDa.
研究背景

NIP30蛋白参与蛋白酶体蛋白分解代谢过程的负调节和蛋白结合活性的负调节。它位于细胞核中。NIP30 蛋白在大脑、睾丸和 25 种其他组织等组织中普遍表达。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

NP_079222 (M1-P254)
基因 ID

80011
蛋白结构
N-term
NIP30 (M1-P254)
Accession # NP_079222
His
C-term
同用名
FAM192A; CDA10; NIP30; CDA018; C16orf94; family with sequence similarity 192, member A; protein FAM192A; NEFA-interacting nuclear protein NIP30
氨基酸序列

MDGGDDGNLIIKKRFVSEAELDERRKRRQEEWEKVRKPEDPEECPEEVYDPRSLYERLQEQKDRKQQEYEEQFKFKNMVRGLDEDETNFLDEVSRQQELIEKQRREEELKELKEYRNNLKKVGISQENKKEVEKKLTVKPIETKNKFSQAKLLAGAVKHKSSESGNSVKRLKPDPEPDDKNQEPSSCKSLGNTSLSGPSIHCPSAAVCIGILPGLGAYSGSSDSESSSDSEGTINATGKIVSSIFRTNTFLEAP

分子量

Approximately 35-43 kDa due to the glycosylation.
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCL, pH 7.3, 10% Glycerol.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料
产品使用指南 (1213 KB)

NIP30 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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