1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. NIT2 蛋白, Arabidopsis thaliana (His)

NIT2 蛋白, Arabidopsis thaliana (His)

目录号: HY-P701989
产品使用指南

NIT2蛋白具有催化3-吲哚乙腈转化为3-吲哚乙酸的非凡能力,3-吲哚乙酸是一种参与许多生理过程的重要植物激素。这一转变意义重大,因为它代表了吲哚-3-乙酸生物合成的关键步骤,吲哚-3-乙酸是植物生长和发育的关键调节剂。NIT2 蛋白, Arabidopsis thaliana (His) 是重组的 NIT2 蛋白,由 E. coli 表达,带有 N-6*His 标签。NIT2 蛋白, Arabidopsis thaliana (His) 全长 339 个氨基酸。

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  • 生物活性

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描述

The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana (His) is the recombinant NIT2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of NIT2 Protein, Arabidopsis thaliana (His) is 339 a.a., .

研究背景

NIT2蛋白具有催化3-吲哚乙腈转化为3-吲哚-乙酸的非凡能力,吲哚-3-乙酸是参与许多生理过程的重要植物激素。这种转变意义重大,因为它代表了吲哚-3-乙酸生物合成的关键步骤,吲哚-3-乙酸是植物生长和发育的关键调节剂。NIT2 的酶活性有助于吲哚-3-乙酸水平的稳态,进而影响植物器官发生、细胞伸长、根发育和对环境刺激的反应。了解 NIT2 蛋白在此代谢途径中的作用和调节可以深入了解植物激素信号传导,并有可能用于农业应用,例如提高作物产量或抗逆性。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

P32962 (M1-K339)

基因 ID

823555

蛋白结构
N-term
6*His
NIT2 (M1-K339)
Accession # P32962
C-term
同用名
NIT2; Nitrilase 2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NIT2 Protein, Arabidopsis thaliana (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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