NKG2A 蛋白, Human (Biotinylated, HEK293, His-Avi)

The NKG2A protein is an important immunosuppressive receptor that forms a complex with KLRD1 on lymphocyte subsets for self-non-self discrimination. It recognizes HLA-E loaded with self-peptides, monitors MHC class I expression in healthy cells, and promotes self-tolerance. NKG2A Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived NKG2A protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of NKG2A Protein, Human (Biotinylated, HEK293, His-Avi) is 134 a.a., with molecular weight of 32-48 kDa.

NKG2A 蛋白是一种对自我-非自我辨别至关重要的免疫抑制受体，与细胞毒性和调节性淋巴细胞亚群上的 KLRD1 形成复合物，识别装载有来自信号的自身肽的非经典主要组织相容性 (MHC) Ib 类分子 HLA-E经典 MHC Ia 类分子的序列。这种识别使细胞毒性细胞能够监测健康细胞中 MHC I 类的表达并促进自我耐受。与 HLA-E 肽复合物结合后，NKG2A 通过两个基于免疫受体酪氨酸的抑制基序 (ITIM) 传递细胞内信号，招募 INPP5D/SHP-1 和 INPPL1/SHP-2 酪氨酸磷酸酶来对抗来自激活受体的信号。作为自然杀伤 (NK) 细胞的关键抑制性受体，NKG2A 调节其激活和效应功能，对抗记忆/效应 CD8 阳性 T 细胞子集上的 T 细胞受体信号传导，并将无害抗原与致病抗原区分开来。在富含 HLA-E 的肿瘤微环境中，NKG2A 充当免疫抑制检查点，导致 NK 细胞和肿瘤特异性 T 细胞效应功能逐渐丧失，这种现象称为细胞衰竭。值得注意的是，在病毒感染期间，NKG2A 识别与人巨细胞病毒衍生肽复合的 HLA-E，抑制 NK 细胞的细胞毒性并促进病毒免疫逃逸。

Immobilized Anti-NKG2A Antibody hFc at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Human NKG2A His with the EC₅₀ of 0.21-0.23 μg/ml determined by ELISA.

Human

HEK293

N-His;N-Avi

P26715-1 (R100-L233)

3821  [NCBI]

RHNNSSLNTRTQKARHCGHCPEEWITYSNSCYYIGKERRTWEESLLACTSKNSSLLSIDNEEEMKFLSIISPSSWIGVFRNSSHHPWVTMNGLAFKHEIKDSDNAELNCAVLQVNRLKSAQCGSSIIYHCKHKL

32-48 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.