1. 重组蛋白
  2. CAR-T related Proteins CD Antigens
  3. Natural Killer Group 2, Member D (NKG2D) T Cell CD Proteins NK Cell CD Proteins
  4. CD314/NKG2D
  5. NKG2D/CD314 蛋白, Cynomolgus (HEK293, His)

NKG2D/CD314 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P72504
COA 产品使用指南

NKG2D/CD314 蛋白是免疫监视中重要的激活和共刺激受体。它与肿瘤和病毒感染细胞上的应激诱导配体结合,激活 NK 细胞,并作为 CD8(+) T 细胞介导的适应性反应的共刺激受体,从而增强 T 细胞活化。NKG2D/CD314 蛋白, Cynomolgus (HEK293, His) 是重组的 NKG2D/CD314 蛋白,由 HEK293 表达,带有 N-6*His 标签。NKG2D/CD314 蛋白, Cynomolgus (HEK293, His) 全长 139 个氨基酸,分子量为 22-35 kDa。

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10 μg ¥1250 In-stock
50 μg ¥3500 In-stock
100 μg   询价  

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描述

The NKG2D/CD314 protein serves as an important activating and costimulatory receptor in immune surveillance. It binds to stress-inducing ligands on tumor and virus-infected cells, activates NK cells and serves as a costimulatory receptor for CD8(+) T cell-mediated adaptive responses, thereby enhancing T cell activation. NKG2D/CD314 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived NKG2D/CD314 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of NKG2D/CD314 Protein, Cynomolgus (HEK293, His) is 139 a.a., with molecular weight of 22-35 kDa.

研究背景

NKG2D/CD314 蛋白作为一种对免疫监视至关重要的激活和共刺激受体,与自体肿瘤细胞和病毒感染细胞上存在的多种应激诱导配体结合。它在先天免疫反应中发挥双重作用,既刺激激活杀伤 (NK) 细胞,又在 CD8(+) T 细胞介导的适应性免疫反应中充当 T 细胞受体 (TCR) 的共刺激受体,从而增强 T 细胞激活。该受体促进穿孔素介导的配体表达肿瘤细胞的消除,并触发涉及钙流入的信号级联反应,最终导致 TNF-α 表达。此外,NKG2D/CD314 参与 NK 细胞介导的骨髓移植排斥反应,并可能调节 NK 细胞的分化和存活。其配体结合能力延伸至 MHC I 类相关糖蛋白的各个亚家族。该蛋白通过二硫键连接形成同二聚体,并与 HCST/DAP10 亚基形成异六聚体,这是 NK 细胞表面表达和细胞毒性诱导的关键相互作用。此外,它可以与 CD94 建立二硫键异二聚体,并与 CEACAM1 相互作用,招募 PTPN6 进行 VAV1 去磷酸化。

种属

Cynomolgus

表达系统

HEK293

标签

N-6*His

蛋白编号

P61252 (F78-V216)

基因 ID

102120479  [NCBI]

蛋白结构
N-term
6*His
NKG2D (F78-V216)
Accession # P61252
C-term
同用名
NKG2-D type II integral membrane protein; CD314; KLRK1; NKG2-D
氨基酸序列

FLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFNESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWEDGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSIPNTYICMQRTV

分子量

22-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NKG2D/CD314 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P72504
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