1. 重组蛋白
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  3. NODAL 蛋白, Human

节点蛋白在胚胎发育中起着关键作用,对于中胚层形成和轴向模式不可或缺。作为通过二硫键连接在一起的同型二聚体,Nodal 有助于形成控制基本发育途径的分子框架,确保胚胎发生过程中中胚层组织和轴向结构的正确建立。NODAL 蛋白, Human 是重组的 NODAL 蛋白,由 E. coli 表达,不带标签。

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规格 价格 是否有货 数量
50 μg ¥14000 1 - 2 周
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Nodal protein plays a key role in embryonic development and is indispensable for mesoderm formation and axial patterning. As a homodimer linked together by disulfide bonds, Nodal helps form the molecular framework that controls fundamental developmental pathways, ensuring the correct establishment of mesodermal tissue and axial structure during embryogenesis. NODAL Protein, Human is the recombinant human-derived NODAL protein, expressed by E. coli, with tag free.

研究背景

Nodal 蛋白在胚胎发育中发挥着关键作用,是中胚层形成和轴向模式不可或缺的因素。作为通过二硫键连接在一起的同型二聚体,Nodal 有助于形成控制基本发育途径的分子框架,确保胚胎发生过程中中胚层组织和轴向结构的正确建立。这种蛋白质不含动物源性成分,强调其适用于研究和生物技术工作中需要严格纯度和道德考虑的应用。

生物活性

Immobilized Human NODAL Protein, Tag Free at 10 μg/mL (100 μL/well) can bind Human SIRP gamma, Fc Tag with a linear range of 0.01-0.625 μg/mL.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q96S42 (H238-L347)

基因 ID

4838

蛋白结构
N-term
NODAL (H238-L347)
Accession # Q96S42
C-term
蛋白长度

Full Length of Mature Protein

中文名
人淋巴结生长分化因子
同用名
HTX5; NODAL; Nodal homolog
氨基酸序列

HLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

预测分子量
12.9 kDa
分子量

Approximately 14-15 kDa, based on SDS-PAGE under reducing conditions.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from 0.22 μm filtered solution in 10 mM Sodium citrate, pH3.0 with trehalose as protectant.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

NODAL Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NODAL Protein, Human
目录号:
HY-P704126
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