NOTCH2 蛋白, Human (HEK293, His)

NOTCH2 protein is a receptor for ligands such as JAG1, JAG2 and DLL1 and is critical for cell fate determination. Ligand activation promotes cleavage and releases NICD, which forms a transcription activator complex with RBPJ/RBPSUH, affecting cell differentiation, proliferation and apoptosis. NOTCH2 Protein, Human (HEK293, His) is the recombinant human-derived NOTCH2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of NOTCH2 Protein, Human (HEK293, His) is 505 a.a., with molecular weight of 60-70 kDa.

NOTCH2 蛋白作为膜结合配体的受体，包括 Jagged-1 (JAG1)、Jagged-2 (JAG2) 和 Delta-1 (DLL1)，在调节细胞命运决定中发挥关键作用。配体激活后，NOTCH2 发生裂解，释放出 Notch 胞内结构域 (NICD)，该结构域与 RBPJ/RBPSUH 形成转录激活因子复合物，并激活分裂位点增强子内的基因。这种激活影响细胞分化、增殖和凋亡程序。NOTCH2 参与骨重塑和体内平衡，与 RELA/p65 合作增强 NFATc1 启动子活性并正向调节 RANKL 诱导的破骨细胞分化。此外，它还积极调节肝癌细胞的自我更新。NOTCH2以异二聚体形式存在，由C端片段(N(TM))和N端片段(N(EC))组成，可能通过二硫键连接。它与多种蛋白质相互作用，包括转录共激活因子 MAML1、MAML2 和 MAML3，以及 HIF1AN、TCIM 和 FBXW7 等因子，表明它参与多种细胞过程和调控途径。与 MINAR1、NOTCH2NL、MDK 和 MINAR2 的相互作用进一步促成了 NOTCH2 信号传导的复杂网络。

Human

HEK293

C-6*His

Q04721 (L26-Q530)

4853  [NCBI]

LQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQ

60-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.