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  3. NRBF2 蛋白, Human (sf9, His-GST)

NRBF2 蛋白, Human (sf9, His-GST)

目录号: HY-P76523
产品使用指南

NRBF2 蛋白可以通过靶核受体调节转录激活,并在体外充当转录激活剂。它参与饥饿诱导的自噬,与 PI3K 复合物 I (PI3KC3-C1) 结合并稳定其组装。NRBF2 蛋白, Human (sf9, His-GST) 是重组的 NRBF2 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。NRBF2 蛋白, Human (sf9, His-GST) 全长 287 个氨基酸,分子量约为 ~61 KDa。

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描述

NRBF2 protein may regulate transcriptional activation through target nuclear receptors and act as a transcriptional activator in vitro. It participates in starvation-induced autophagy, binding to PI3K complex I (PI3KC3-C1) and stabilizing its assembly. NRBF2 Protein, Human (sf9, His-GST) is the recombinant human-derived NRBF2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of NRBF2 Protein, Human (sf9, His-GST) is 287 a.a., with molecular weight of ~61 KDa.

研究背景

NRBF2 蛋白可能通过靶核受体调节转录激活,在体外充当转录激活剂。它还参与饥饿诱导的自噬,可能是通过其与 PI3K 复合物 I (PI3KC3-C1) 的关联,尽管其作用的描述各异。NRBF2 与饥饿诱导的自噬的诱导、稳定 PI3KC3-C1 组装并增强 PIK3C3 的 ATG14 连接脂质激酶活性相关。此外,它还可以负向调节基础自噬和饥饿诱导的自噬,通过调节 PI3KC3-C1 的相互作用来抑制 PIK3C3 活性。此外,NRBF2 可能参与自噬体生物发生,并在神经祖细胞分化过程中的存活中发挥作用,强调其在细胞过程中的多方面功能。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q96F24 (M1-N287)

基因 ID
蛋白结构
N-term
His-GST
NRBF2 (M1-N287)
Accession # Q96F24
C-term
同用名
Nuclear receptor-binding factor 2; Comodulator of PPAR and RXR; COPR
分子量

Approximately 61 kDa.

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NRBF2 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NRBF2 Protein, Human (sf9, His-GST)
目录号:
HY-P76523
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