NRCAM 蛋白, Human (HEK293, Fc)

Neuronal cell adhesion molecule (NRCAM) is a member of the immunoglobulin superfamily and a cell adhesion protein. NRCAM is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system while being crucial for the saltatory propagation of action potentials along myelinated axons. NRCAM also plays a general role in cell-cell communication. NRCAM Protein, Human (HEK293, Fc) is the recombinant human-derived NRCAM protein, expressed by HEK293 , with C-hFc labeled tag. The total length of NRCAM Protein, Human (HEK293, Fc) is 576 a.a., with molecular weight of ~114.0 kDa.

神经细胞粘附分子 (NRCAM) 是免疫球蛋白超家族的成员，是一种细胞粘附蛋白，具有多个免疫球蛋白样 C2 型结构域和纤连蛋白 III 型结构域。NRCAM 作为锚蛋白结合蛋白参与神经元-神经元粘附，并在轴突锥生长过程中促进定向信号传导。NRCAM 在介导雪旺细胞和轴突之间的细胞间接触、髓鞘轴突 Ranvier 结的形成和维持、钠通道在半结处的正常聚集等作用中发挥作用。因此，NRCAM 是大脑和周围神经系统中对细胞间接触的正常反应所必需的，同时也是动作电位沿有髓鞘轴突跳跃传播的关键。NRCAM 也在非神经组织中表达，并可能在细胞定向迁移过程中通过胞内结构域向肌动蛋白骨架传递信号，在细胞间通讯中发挥普遍作用。NRCAM 等位基因变异与自闭症和成瘾脆弱性有关^[1][2][3]。

Human

HEK293

C-hFc

AAI15737.1 (Q25-N600)

4897  [NCBI]

QMISALEVPLDPKLLEDLVQPPTITQQSPKDYIIDPRENIVIQCEAKGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSPLWTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPEDTREDYICYARFNHTQTIQQKQPISVKVISAKSSRERPPTFLTPEGNASNKEELRGNVLSLECIAEGLPTPIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVRVKAAPYWITAPQNLVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFSNVQERSSAVYQCNASNEYGYLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFKGAKGSALHEDIYVLHENGTLEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDATWIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVAN

Approximately 114.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.