1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Neuregulins
  5. Neuregulin-1 (NRG1)
  6. NRG1-beta 1 蛋白, Human (246a.a, His)

NRG1-beta 1 蛋白, Human (246a.a, His)

目录号: HY-P70967
COA 产品使用指南

NRG1 是 ERBB3 和 ERBB4 酪氨酸激酶受体的直接配体。NRG1 可诱导 MAPK3/ERK1MAPK1/ERK2AKT1 的磷酸化和激活。NRG1 可诱导细胞生长、分化和增殖。NRG1-beta 1 蛋白, Human (246a.a, His) 是重组的 NRG1-β 1 蛋白,由 E. coli 表达,带有 N-6*His 标签。NRG1-beta 1 蛋白, Human (246a.a, His) 全长 246 个氨基酸,分子量约为 ~35.0 kDa。

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描述

NRG1 is a direct ligand of the ERBB3 and ERBB4 tyrosine kinase receptors. NRG1 can induce the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. NRG1 can induce cell growth, differentiation and proliferation. NRG1-beta 1 Protein, Human (246a.a, His) is the recombinant human-derived NRG1-beta 1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of NRG1-beta 1 Protein, Human (246a.a, His) is 246 a.a., with molecular weight of ~35.0 kDa.

研究背景

Pro-neuregulin-1, membrane-bound isoform (NRG1) 是 ERBB3 和 ERBB4 酪氨酸激酶受体的直接配体。NRG1 的多种异构体可诱导上皮细胞、胶质细胞、神经元细胞和骨骼肌细胞的生长和分化;神经肌肉接点形成过程中突触囊泡中乙酰胆碱受体的诱导表达刺激乳腺小叶肺泡萌芽和泌乳,诱导乳腺肿瘤细胞分化;刺激雪旺细胞增殖;影响心肌的发育。NRG1 也是整合素的配体并结合 (通过 EGF 结构域) 整合素 ITGAV:ITGB3 或 ITGA6:ITGB4。NRG1 可诱导MAPK3/ERK1MAPK1/ERK2AKT1 的磷酸化和激活。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

AAA58639.1 (M1-K246)

基因 ID

3084  [NCBI]

蛋白结构
N-term
6*His
NRG1-beta 1 (M1-K246)
Accession # AAA58639.1
C-term
中文名
重组人NRG1-beta 1蛋白
同用名
Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1 ECD; NRG1; GGF; HGL; HRGA; NDF; SMDF;
氨基酸序列

MSERKEGRGKGKGKKKERGSGKKPESAAGSQSPALPPQLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK

分子量

Approximately 35.0 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

NRG1-beta 1 Protein, Human (246a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NRG1-beta 1 Protein, Human (246a.a, His)
目录号:
HY-P70967
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