Nucleophosmin/Npm1 蛋白, Mouse (His-SUMO)

The nucleophosmin/Npm1 protein is a multifunctional nucleolar phosphoprotein involved in multiple cellular processes, including ribosome assembly and transport, DNA repair, and centrosome duplication. It plays a crucial role in maintaining genome stability and regulating cell proliferation. Nucleophosmin/Npm1 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Nucleophosmin/Npm1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Nucleophosmin/Npm1 Protein, Mouse (His-SUMO) is 292 a.a., with molecular weight of ~48.6 kDa.

Nucleophosmin/Npm1 是一种多功能蛋白，参与多种细胞过程，包括核糖体生物发生、中心体复制、蛋白伴侣、组蛋白组装、细胞增殖以及 p53/TP53 和 ARF 等肿瘤抑制因子的调节。它参与驱动核糖体核输出与其与核糖体的结合有关，同时它与核仁核糖核蛋白结构形成复合物并结合单链核酸。Npm1 作为核心组蛋白 H3、H2B 和 H4 的伴侣蛋白，可刺激双链 DNA 上的 APEX1 核酸内切酶活性，但抑制单链 RNA 上的 APEX1 核酸内切酶活性。此外，它还在控制核仁内 APEX1 核酸内切酶活性方面发挥作用，有助于修复 rDNA 上的无嘌呤/无嘧啶位点以及去除氧化的 rRNA 分子。Npm1 参与中心体和中心粒复制，负向调节 EIF2AK2/PKR 激活以抑制细胞凋亡。此外，它还与各种蛋白质相互作用，例如 MYC、NSUN2、SENP3 和 NEK2，突出了其多样化的分子伙伴关系和功能多样性。该蛋白质在特定条件下与二硫键连接的二聚体形成十聚体，并参与 SWAP 复合物等复合物，强调其在细胞过程中的动态参与。

Mouse

E. coli

N-His;N-SUMO

Q61937 (1M-292L)

18148  [NCBI]

MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDEDDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL

Approximately 48.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.