1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Nucleoprotein
  5. Nucleoprotein/NP 蛋白, Influenza B (EPI1368865, sf9, His)

Nucleoprotein/NP 蛋白, Influenza B (EPI1368865, sf9, His)

目录号: HY-P74667
产品使用指南

流感病毒核蛋白 (NP) 是一种包衣病毒负链 RNA 的结构蛋白。NP 蛋白在病毒生命周期中保护病毒 RNA 不被细胞酶降解、拟合 RNP 的螺旋结构、以组蛋白样方式调节病毒 RNA 模板的转录和复制以及在感染时诱导免疫抑制。Nucleoprotein/NP 蛋白, Influenza B (EPI1368865, sf9, His) 是重组的 核蛋白/NP 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。Nucleoprotein/NP 蛋白, Influenza B (EPI1368865, sf9, His) 全长 560 个氨基酸,分子量约为 ~63 kDa。

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描述

Influenza virus nucleoprotein (NP) is a structural protein that coats viral negative-strand RNA. NP proteins protect viral RNA from degradation by cellular enzymes during the viral life cycle, fit the helical structure of RNP, regulate transcription and replication of viral RNA templates in a histone-like manner, and induce immunosuppression during infection. Nucleoprotein/NP Protein, Influenza B (EPI1368865, sf9, His) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Nucleoprotein/NP Protein, Influenza B (EPI1368865, sf9, His) is 560 a.a., with molecular weight of ~63 kDa.

研究背景

流感病毒核蛋白 (NP) 是一种包衣病毒负链 RNA 的结构蛋白。NP 是物种特异性的主要决定因素之一。NP 基因在多大程度上可以通过重组跨越物种屏障,并通过突变适应新的宿主。NP 蛋白在病毒生命周期中保护病毒 RNA 不被细胞酶降解、拟合 RNP 的螺旋结构、以组蛋白样方式调节病毒 RNA 模板的转录和复制以及在感染时诱导免疫抑制 (抑制效应细胞因子合成和 FcR 药物信号传导)。NP 是未来疫苗开发的一个非常有前途的目标。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

EPI1368865 (M1-Y560)

基因 ID

/

蛋白结构
N-term
Nucleoprotein (M1-Y560)
Accession # EPI1368865
His
C-term
同用名
Influenza B (B/Washington/02/2019) Nucleoprotein / NP Protein (sf9, His)
分子量

Approximately 63 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Nucleoprotein/NP Protein, Influenza B (EPI1368865, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Nucleoprotein/NP Protein, Influenza B (EPI1368865, sf9, His)
目录号:
HY-P74667
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