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  3. O-GlcNAcase/OGA 蛋白, B. thetaiotaomicron (His)

O-GlcNAcase/OGA 蛋白, B. thetaiotaomicron (His)

目录号: HY-P79242
COA 产品使用指南 技术支持

O-GlcNAcase (OGA) 是一种负责水解 O-GlcNA 酰化蛋白中糖苷键的酶。它通过有效利用对硝基苯基-β-GlcNAc 和 4-甲基伞形酮-GlcNAc 等底物在体外表现出其酶活性。O-GlcNAcase/OGA 蛋白, B. thetaiotaomicron (His) 是重组的 O-GlcNAcase/OGA 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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规格 价格 是否有货 数量
5 μg ¥1350 In-stock
10 μg ¥2300 In-stock
50 μg ¥6500 In-stock
100 μg ¥11050 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

O-GlcNAcase (OGA) is an enzyme responsible for the hydrolysis of glycosidic bonds in O-GlcNA acylated proteins. It exhibits its enzymatic activity in vitro by efficiently utilizing substrates such as p-nitrophenyl-β-GlcNAc and 4-methylumbelliferone-GlcNAc. O-GlcNAcase/OGA Protein, B. thetaiotaomicron (His) is the recombinant O-GlcNAcase/OGA protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

O-GlcNAcase (OGA) 是一种负责 O-GlcNA 酰化蛋白中糖苷键水解的酶。它通过有效利用对硝基苯基-β-GlcNAc 和 4-甲基伞形酮-GlcNAc 等底物在体外展示其酶活性。OGA 在调节 O-GlcNAc 酰化中发挥着至关重要的作用,O-GlcNAc 酰化是一种翻译后修饰,涉及 N-乙酰氨基葡萄糖 (GlcNAc) 与蛋白质的丝氨酸或苏氨酸残基的连接。通过催化 O-GlcNAc 基团的去除,OGA 有助于动态控制 O-GlcNAc 化水平,从而影响各种细胞过程,如信号转导、基因表达和蛋白质稳定性。为了充分理解 OGA 在细胞功能和疾病途径中的复杂调节机制和生理意义,需要进一步研究。

生物活性

Measured by its ability to cleave a peptide substrate, 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide. Read at excitation and emission wavelengths of 365 nm and 445 nm. The specific activity is 3120.1541 pmol/min/µg, as measured under the described conditions.

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

Q89ZI2 (Q22-K737)

基因 ID

1074035

蛋白结构
N-term
6*His
OGA (Q22-K737)
Accession # Q89ZI2
C-term
蛋白长度

Full Length of Mature Protein

中文名
O-GlcNAcase 蛋白/OGA 蛋白
同用名
O-GlcNAcase BT_4395; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Beta-hexosaminidase; Hexosaminidase B; N-acetyl-beta-glucosaminidase
氨基酸序列

QNVSLQPPPQQLIVQNKTIDLPAVYQLNGGEEANPHAVKVLKELLSGKQSSKKGMLISIGEKGDKSVRKYSRQIPDHKEGYYLSVNEKEIVLAGNDERGTYYALQTFAQLLKDGKLPEVEIKDYPSVRYRGVVEGFYGTPWSHQARLSQLKFYGKNKMNTYIYGPKDDPYHSAPNWRLPYPDKEAAQLQELVAVANENEVDFVWAIHPGQDIKWNKEDRDLLLAKFEKMYQLGVRSFAVFFDDISGEGTNPQKQAELLNYIDEKFAQVKPDINQLVMCPTEYNKSWSNPNGNYLTTLGDKLNPSIQIMWTGDRVISDITRDGISWINERIKRPAYIWWNFPVSDYVRDHLLLGPVYGNDTTIAKEMSGFVTNPMEHAESSKIAIYSVASYAWNPAKYDTWQTWKDAIRTILPSAAEELECFAMHNSDLGPNGHGYRREESMDIQPAAERFLKAFKEGKNYDKADFETLQYTFERMKESADILLMNTENKPLIVEITPWVHQFKLTAEMGEEVLKMVEGRNESYFLRKYNHVKALQQQMFYIDQTSNQNPYQPGVKTATRVIKPLIDRTFATVVKFFNQKFNAHLDATTDYMPHKMISNVEQIKNLPLQVKANRVLISPANEVVKWAAGNSVEIELDAIYPGENIQINFGKDAPCTWGRLEISTDGKEWKTVDLKQKESRLSAGLQKAPVKFVRFTNVSDEEQQVYLRQFVLTIEKK

分子量

80-95 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

O-GlcNAcase/OGA Protein, B. thetaiotaomicron (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
O-GlcNAcase/OGA Protein, B. thetaiotaomicron (His)
目录号:
HY-P79242
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