OGT 蛋白, Human (His)

OGT Protein, Human (His) is the recombinant human-derived OGT protein, expressed by E. coli, with N-6*His & N-SUMO tag.

OGT 是一种催化酶，能够将单个 N-acetylglucosamine 从 UDP-GlcNAc 转移到细胞质和核蛋白中的丝氨酸或苏氨酸残基上，从而通过 β-连接的 N-acetylglucosamine (O-GlcNAc) 修饰这些蛋白质。它糖基化大量多样的蛋白质，包括 histone H2B、AKT1、AMPK、ATG4B、CAPRIN1、EZH2 等，并通过糖基化与磷酸化的交互作用或影响蛋白水解过程来调控它们的细胞功能。在肌肉和脂肪细胞中，OGT 通过糖基化胰岛素信号组分并抑制 AKT1 的 'Thr-308' 磷酸化来参与胰岛素抵抗 (by similar)。此外，OGT 通过介导 6-phosphofructokinase PFKL 的糖基化来调节糖酵解，抑制其活性。在染色质结构中，OGT 通过介导 histone H2B 的 'Ser-112' O-GlcNAcylation 发挥关键作用，并通过与 TET 蛋白 (TET1、TET2 或 TET3) 的相互作用被招募到活跃基因的 CpG 富集转录起始位点。线粒体亚型 (mOGT) 具有细胞毒性，可在多种细胞类型 (包括胰岛素瘤细胞系 INS1) 中触发凋亡。

Human

E. coli

N-6*His;N-SUMO

O15294-1 (M606-Q1022)

8473

Partial

MAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQ

Approximately 68 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.