OLR1 蛋白, Human (HEK293, His)

OLR1 Protein, a receptor on vascular endothelial cells, plays a key role in recognizing, internalizing, and degrading oxidatively modified low-density lipoprotein (oxLDL). As a marker of atherosclerosis, oxLDL induces vascular endothelial cell activation, leading to pro-inflammatory responses, oxidative conditions, and apoptosis. OLR1's association with oxLDL activates NF-kappa-B, contributing to pro-atherogenic responses such as reduced nitric oxide release, monocyte adhesion, and apoptosis. Beyond oxLDL, it serves as a receptor for HSP70, participating in cell-mediated antigen cross-presentation and functioning as a leukocyte-adhesion molecule in inflammation. Additionally, OLR1 recognizes AGE products, activated platelets, monocytes, apoptotic cells, and bacteria. OLR1 Protein, Human (HEK293, His) is the recombinant human-derived OLR1 protein, expressed by HEK293, with C-6*His labeled tag. The total length of OLR1 Protein, Human (HEK293, His) is 213 a.a., with molecular weight of 30-35 kDa.

OLR1 是一种关键受体，在介导血管内皮细胞识别、内化和降解氧化修饰低密度脂蛋白 (oxLDL) 方面发挥着至关重要的作用。oxLDL 与 OLR1 的结合触发血管内皮细胞激活和功能障碍，导致促炎症反应、氧化条件增加和细胞凋亡，凸显了其作为动脉粥样硬化标志物的重要性。这种与 oxLDL 的相互作用会激活 NF-kappa-B，导致细胞内活性氧产生增加和一系列促动脉粥样硬化细胞反应，包括一氧化氮释放减少、单核细胞粘附增加和细胞凋亡。除了参与动脉粥样硬化之外，OLR1 还充当 HSP70 蛋白的受体，有助于抗原交叉呈递给树突状细胞中的幼稚 T 细胞，并参与细胞介导的抗原交叉呈递。此外，OLR1在内毒素诱导的炎症期间作为血管界面处的白细胞粘附分子发挥作用，并作为晚期糖基化终产物、活化血小板、单核细胞、凋亡细胞以及革兰氏阴性和革兰氏阳性细菌的受体。在微生物感染的情况下，OLR1 可能充当脑膜炎奈瑟菌粘附素 A 变体 3 (nadA) 的受体。

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 0.797 μg/ml, corresponding to a specific activity is 1.25×10^3 units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 0.797 μg/ml, corresponding to a specific activity is 1.25×10³ units/mg.

Human

HEK293

C-6*His

P78380 (S61-Q273)

4973  [NCBI]

SQVSDLLTQEQANLTHQKKKLEGQISARQQAEEASQESENELKEMIETLARKLNEKSKEQMELHHQNLNLQETLKRVANCSAPCPQDWIWHGENCYLFSSGSFNWEKSQEKCLSLDAKLLKINSTADLDFIQQAISYSSFPFWMGLSRRNPSYPWLWEDGSPLMPHLFRVRGAVSQTYPSGTCAYIQRGAVYAENCILAAFSICQKKANLRAQ

30-35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.