OMA1 蛋白, Human (Cell-Free, His-SUMO)

Oma1 protein is an important metalloprotease in the inner mitochondrial membrane that is activated in response to stressors and cleaves targets such as OPA1, UQCC3, and DELE1. Under conditions of loss of membrane potential, Oma1 cleaves OPA1, thereby negatively regulating fusion. OMA1 Protein, Human (Cell-Free, His-SUMO) is the recombinant human-derived OMA1 protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag. The total length of OMA1 Protein, Human (Cell-Free, His-SUMO) is 511 a.a., with molecular weight of ~74.7 kDa.

Oma1 蛋白被归类为金属蛋白酶，在线粒体内膜内发挥作用，是质量控制系统的重要组成部分。为了响应不同的线粒体应激源，OMA1 被激活，导致特定靶蛋白（包括 OPA1、UQCC3 和 DELE1）的蛋白水解裂解。在导致线粒体膜电位丧失的情况下，OMA1 在 S1 位置裂解 OPA1，导致 OPA1 失活和线粒体融合的负调节。此外，OMA1 作为细胞凋亡的关键调节因子，通过 BAK 和 BAX 聚集时 OPA1 裂解促进线粒体嵴重塑，从而释放细胞色素 c。在去极化线粒体中，OMA1 可能充当 PINK1 的备用蛋白酶，介导其裂解以及随后被蛋白酶体降解。此外，OMA1 参与整合应激反应 (ISR)，裂解 DELE1 生成加工形式 (S-DELE1)，后者易位到细胞质并激活 EIF2AK1/HRI，启动 ISR。OMA1 在线粒体质量控制中的作用延伸到脂质代谢的调节、呼吸超复合物稳定性的维持以及通过影响体温和能量消耗来适应冷应激条件。它与心磷脂的结合表明其在调节心磷脂周转方面具有潜在作用。

Measured by its binding ability in a functional ELISA. Immobilized OMA1 at 5 μg/mL can bind human BZW2, the EC₅₀ of human BZM2 is 10.108-22.636 μg/mL.

Human

E. coli Cell-free

N-His;N-SUMO

Q96E52 (H14-S524)

115209  [NCBI]

HVFFRFNSLSNWRKCNTLASTSRGCHQVQVNHIVNKYQGLGVNQCDRWSFLPGNFHFYSTFNNKRTGGLSSTKSKEIWRITSKCTVWNDAFSRQLLIKEVTAVPSLSVLHPLSPASIRAIRNFHTSPRFQAAPVPLLLMILKPVQKLFAIIVGRGIRKWWQALPPNKKEVVKENIRKNKWKLFLGLSSFGLLFVVFYFTHLEVSPITGRSKLLLLGKEQFRLLSELEYEAWMEEFKNDMLTEKDARYLAVKEVLCHLIECNKDVPGISQINWVIHVVDSPIINAFVLPNGQMFVFTGFLNSVTDIHQLSFLLGHEIAHAVLGHAAEKAGMVHLLDFLGMIFLTMIWAICPRDSLALLCQWIQSKLQEYMFNRPYSRKLEAEADKIGLLLAAKACADIRASSVFWQQMEFVDSLHGQPKMPEWLSTHPSHGNRVEYLDRLIPQALKIREMCNCPPLSNPDPRLLFKLSTKHFLEESEKEDLNITKKQKMDTLPIQKQEQIPLTYIVEKRTGS

Approximately 74.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.