1. 重组蛋白
  2. Others
  3. Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO)

Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO)

目录号: HY-P71493
COA 产品使用指南

外膜蛋白 A (OmpA) 确保细菌细胞壁的结构完整性,并与 TolR 配合稳定肽聚糖层。作为孔蛋白,OmpA 控制小溶质的渗透性。Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO) 是重组的 外膜蛋白 A/OmpA 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO) 全长 183 个氨基酸,分子量约为 ~35.6 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
5 μg ¥1400 In-stock
10 μg ¥2360 In-stock
50 μg ¥6600 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Outer membrane protein A (OmpA) ensures the structural integrity of the bacterial cell wall and cooperates with TolR to stabilize the peptidoglycan layer. As a porin, OmpA controls the permeability of small solutes. Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Outer membrane protein A/OmpA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) is 183 a.a., with molecular weight of ~35.6 kDa.

研究背景

外膜蛋白 A (OmpA) 在细菌细胞壁的结构完整性中发挥着至关重要的作用,与 TolR 协同作用以维持肽聚糖层在周质中的位置。作为孔蛋白,OmpA 表现出低渗透性,有助于小溶质逐渐渗透其结构。OmpA 内的内门进一步调节溶质的通过,影响分子的选择性运输。此外,OmpA 的重要性还在于它参与与 F 型质粒的接合,它可能作为受体细胞上的重要交配受体,有助于遗传物质的水平转移。

种属

E.coli

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P0A911 (W164-A346)

基因 ID

917123  [NCBI]

蛋白结构
N-term
6*His-SUMO
OmpA (W164-A346)
Accession # P0A911
C-term
同用名
ompA; Z1307; ECs1041; Outer membrane protein A; Outer membrane porin A
氨基酸序列

WTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA

分子量

Approximately 35.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Outer membrane protein A/OmpA Protein, E.coli (His-SUMO)
目录号:
HY-P71493
需求量: