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Outer membrane 蛋白 F/OmpF 蛋白, E.coli (His, myc)

目录号: HY-P72279
COA 产品使用指南

外膜蛋白 F (OmpF) 促进孔的形成,使小分子能够被动扩散穿过细菌外膜。除了在膜通透性方面的作用之外,OmpF 还作为噬菌体 T2 的重要受体,并被认为是大肠菌素 E5 的主要受体,在与这些相互作用相关的机制中可能发挥着作用。Outer membrane 蛋白 F/OmpF 蛋白, E.coli (His, myc) 是重组的 外膜蛋白 F/OmpF 蛋白,由 E. coli 表达,带有 C-Myc, N-10*His 标签。Outer membrane 蛋白 F/OmpF 蛋白, E.coli (His, myc) 全长 340 个氨基酸,分子量约为 ~44.1 kDa。

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描述

Outer membrane protein F (OmpF) facilitates the formation of pores, enabling the passive diffusion of small molecules across the bacterial outer membrane. Beyond its role in membrane permeability, OmpF serves as a crucial receptor for the bacteriophage T2 and is considered the primary receptor for colicin E5, playing a probable role in the mechanisms associated with these interactions. Outer membrane protein F/OmpF Protein, E.coli (His, myc) is the recombinant E. coli-derived Outer membrane protein F/OmpF protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of Outer membrane protein F/OmpF Protein, E.coli (His, myc) is 340 a.a., with molecular weight of ~44.1 kDa.

研究背景

外膜蛋白 F (OmpF) 在细菌生理学中发挥着至关重要的作用,因为它形成孔,促进小分子穿过外膜的被动扩散。OmpF 作为膜渗透性的关键组成部分,有助于细菌细胞吸收必需的营养物质和物质。此外,OmpF 作为噬菌体 T2 的受体,使病毒能够识别并感染细菌宿主。此外,OmpF 和大肠杆菌素 E5 之间可能存在关联,其中 OmpF 被确定为该细菌素的主要受体。OmpF 在营养吸收及其与噬菌体和大肠菌素相互作用方面的双重功能强调了其在细菌膜动力学和宿主防御机制中的重要性。

种属

E.coli

表达系统

E. coli

标签

C-Myc;N-10*His

蛋白编号

P02931 (A23-F362)

基因 ID

61754051/945554  [NCBI]

蛋白结构
N-term
10*His
OmpF (A23-F362)
Accession # P02931
C-term
同用名
Outer membrane protein B; Outer membrane protein IA
氨基酸序列

AEIYNKDGNKVDLYGKAVGLHYFSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNKTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADRTNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF

分子量

Approximately 44.1 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1.0 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Outer membrane protein F/OmpF Protein, E.coli (His, myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Outer membrane protein F/OmpF Protein, E.coli (His, myc)
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HY-P72279
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