1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory immune checkpoint molecules Macrophage CD Proteins
  4. TNF Superfamily Ligands OX40 Ligand/CD252
  5. OX40 Ligand
  6. OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His-Flag)

OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His-Flag)

目录号: HY-P78504
产品使用指南

OX40 Ligand (TNFSF4) 是一种 II 型糖蛋白,其结构由含 23 个氨基酸的胞质尾区和含 133 个氨基酸的胞外结构域组成。OX40 Ligand 是 TNFRSF4 (CD134) 的配体,属于肿瘤坏死因子 (TNF) 家族。OX40 Ligand 可以激活 OX40,从而扮演 T 细胞共刺激分子的角色。OX40-OX40 Ligand 之间的相互作用可促进效应 T 细胞存活并有效地诱导记忆 T 细胞生成,以及增强 Tfh 细胞对 B 细胞的辅助功能、促进DCs的分化和成熟。OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His-Flag) 是一种由 HEK293 产生的重组人 OX40 Ligand (Q51-L183),N 端带 His 和 Flag 标签。

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描述

OX40 Ligand (TNFSF4) is a type II glycoprotein with a cytoplasmic tail of 23 aa and an extracellular domain of 133 aa[1]. OX40 Ligand is a ligand for TNFRSF4 (CD134), belongs to tumor necrosis factor (TNF) family. OX40 Ligand can activate OX40 and thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs[1][2]. OX40 Ligand/TNFSF4 Protein, Human (HEK293, His-Flag) is a recombinant human OX40 Ligand (Q51-L183) with N-terminal His and Flag tag, which is produced in HEK293.

研究背景

OX40 Ligand (TNFSF4) is a type II glycoprotein with a cytoplasmic tail of 23 aa and an extracellular domain of 133 aa[1]. OX40 Ligand is expressed on antigen-presenting cells, such as B cells, dendritic cells (DCs), and macrophages, and airway smooth muscle cells[3]. OX40 Ligand is a ligand for TNFRSF4 (CD134), belongs to tumor necrosis factor (TNF) family.
OX40 Ligand can activate OX40 and thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs[1][2].
Human OX40 Ligand shares <70% aa sequence identity with mouse, rat and rabbit.
The interaction between OX40 Ligand with OX40 is essential for the generation of antigen-specific memory T cells, and induces host antitumor immunity[4]. But the over-upregulation of OX40 and OX40L may induce abnormal activation of Tfh cells and excessive production of autoantibodies, which leads to autoimmune disease[1].

体外研究
(In Vitro)

OX40 Ligand (human, 100 ng/mL, 7 days, added into DC-T cell cultures at days 0 and 4) inhibits the generation of IL-10-producing T cells induced by immature DCs and DCs treated with IL-10 and IFN-α[5].

生物活性

Immobilized Human OX40 Ligand Trimer His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human OX40 hFc with the EC50 of 0.16 μg/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

N-His;N-Flag

蛋白编号

P23510 (Q51-L183)

中文名
重组人 OX40 配体/TNFSF4 蛋白 (HEK293, His-Flag)
同用名
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;  TXGP1; Glycoprotein Gp34
分子量

65-140 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OX40 Ligand/TNFSF4 Protein, Human (HEK293, His-Flag)
目录号:
HY-P78504
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