1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  4. TNF Receptor Superfamily CD134/OX40
  5. OX40
  6. OX40/TNFRSF4 蛋白, Human (HEK293, His)

OX40/TNFRSF4 蛋白, Human (HEK293, His)

目录号: HY-P7394
COA 产品使用指南

OX40 (TNFRSF4) 是 OX40 配体的受体。OX40 常在 T 细胞上表达。OX40 Ligand 可以激活 OX40,从而扮演 T 细胞共刺激分子的角色。OX40-OX40 Ligand 之间的相互作用可促进效应 T 细胞存活并有效地诱导记忆 T 细胞生成,以及增强 Tfh 细胞对 B 细胞的辅助功能、促进 DCs 的分化和成熟。OX40/TNFRSF4 蛋白, Human (HEK293, His) 是一种由 HEK293 产生的重组人 OX40 (L29-A216),C 端带 His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1150 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

OX40 (TNFRSF4), is a receptor for OX40 Ligand. OX40 is preferentially expressed by T cells. OX40 can be activated by OX40 Ligand, thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs[1][2]. OX40/TNFRSF4 Protein, Human (HEK293, His) is a recombinant human OX40 (L29-A216) with C-terminal His tag, which is produced in HEK293.

研究背景

OX40 (TNFRSF4) 是 TNFR 超家族的成员,是 OX40 配体的受体。OX40优先由T细胞表达,但也在自然杀伤T细胞、自然杀伤细胞、中性粒细胞和人气道平滑肌细胞中发现。人 OX40 与小鼠和大鼠的氨基酸序列同一性<30%。小鼠 OX40 与大鼠[1]有 90% aa 序列同一性。
OX40 配体可以激活 OX40,从而起到 T 细胞共刺激分子的作用。OX40-OX40配体相互作用促进效应T细胞存活并有效诱导记忆T细胞生成,增强Tfh对B细胞的辅助功能,并促进DC的分化和成熟[1]< /sup>[2]
OX40配体与OX40之间的相互作用对于抗原特异性记忆T细胞的产生至关重要,并诱导宿主抗肿瘤免疫[3]。但 OX40 和 OX40L 的过度上调可能会导致 Tfh 细胞异常激活和自身抗体过度产生,从而导致自身免疫性疾病[1]

体外研究
(In Vitro)

OX40 increases expression of RANTES protein in HUVECs[4].

生物活性

1.2 µg/mL (100 µL/well) of immoblized recombinant human OX40/TNFRSF4-His can bind human Biotin-OX40L-His with a linear range of 1.22-19.53 ng/mL.
2.Immobilized Mouse OX40L-His at 10μg/mL (100 μl/well) can bind Human OX40-6His*: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human OX40-6His is <8.33 μg/mL.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P43489 (L29-A216)

基因 ID
蛋白结构
N-term
OX40 (L29-A216)
Accession # P43489
His
C-term
中文名
重组人 OX40/TNFSF4 蛋白 (HEK293, His)
同用名
rHuOX40/TNFRSF4, His; ACT35 antigen; CD134; TXGP1L
氨基酸序列

LHCVGDTYPSNDRCCHECRPGNGMVSRCSRSQNTVCRPCGPGFYNDVVSSKPCKPCTWCNLRSGSERKQLCTATQDTVCRCRAGTQPLDSYKPGVDCAPCPPGHFSPGDNQACKPWTNCTLAGKHTLQPASNSSDAICEDRDPPATQPQETQGPPARPITVQPTEAWPRTSQGPSTRPVEVPGGRAVAHHHHHH

分子量

38-45 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol or PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
OX40/TNFRSF4 Protein, Human (HEK293, His)
目录号:
HY-P7394
需求量: