1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules Macrophage CD Proteins
  4. TNF Superfamily Ligands OX40 Ligand/CD252
  5. OX40 Ligand
  6. OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His)

OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His)

目录号: HY-P70622
COA 产品使用指南

OX40 Ligand (TNFSF4) 是一种 II 型糖蛋白,其结构由含 23 个氨基酸的胞质尾区和含 133 个氨基酸的胞外结构域组成。OX40 Ligand 是 TNFRSF4 (CD134) 的配体,属于肿瘤坏死因子 (TNF) 家族。OX40 Ligand 可以激活 OX40,从而扮演 T 细胞共刺激分子的角色。OX40-OX40 Ligand 之间的相互作用可促进效应 T 细胞存活并有效地诱导记忆 T 细胞生成,以及增强 Tfh 细胞对 B 细胞的辅助功能、促进DCs的分化和成熟。OX40 Ligand/TNFSF4 蛋白, Human (HEK293, His) 是一种由 HEK293 产生的重组人 OX40 Ligand (Q51-L183),N 端带 6*His 标签。

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10 μg ¥1600 In-stock
50 μg ¥4500 询价
100 μg   询价  

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描述

OX40 Ligand (TNFSF4) is a type II glycoprotein with a cytoplasmic tail of 23 aa and an extracellular domain of 133 aa[1]. OX40 Ligand is a ligand for TNFRSF4 (CD134), belongs to tumor necrosis factor (TNF) family. OX40 Ligand can activate OX40 and thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs[1][2]. OX40 Ligand/TNFSF4 Protein, Human (HEK293, His) is a recombinant human OX40 Ligand (Q51-L183) with N-terminal 6*His tag, which is produced in HEK293.

研究背景

OX40 配体 (TNFSF4) 是一种 II 型糖蛋白,具有 23 个氨基酸的胞质尾部和 133 个氨基酸的胞外结构域[1]OX40 配体在抗原呈递细胞上表达,例如 B 细胞、树突状细胞 (DC) 和巨噬细胞以及气道平滑肌细胞[3]OX40 Ligand是TNFRSF4 (CD134)的配体,属于肿瘤坏死因子(TNF)家族。
OX40 Ligand可以激活OX40,从而起到T细胞共刺激分子的作用。OX40-OX40配体相互作用促进效应T细胞存活并有效诱导记忆T细胞生成,增强Tfh对B细胞的辅助功能,并促进DC的分化和成熟[1]< /sup>[2]
人OX40配体与小鼠、大鼠和兔的氨基酸序列一致性<70%。
OX40配体与OX40之间的相互作用对于抗原特异性记忆T细胞的生成至关重要,并诱导宿主抗肿瘤免疫[4]。但 OX40 和 OX40L 的过度上调可能会导致 Tfh 细胞异常激活和自身抗体过度产生,从而导致自身免疫性疾病[1]

体外研究
(In Vitro)

OX40 Ligand (human, 100 ng/mL, 7 days, added into DC-T cell cultures at days 0 and 4) inhibits the generation of IL-10-producing T cells induced by immature DCs and DCs treated with IL-10 and IFN-α[5].

种属

Human

表达系统

HEK293

标签

N-6*His

蛋白编号

P23510 (Q51-L183)

基因 ID
蛋白结构
N-term
6*His
OX40L (Q51-L183)
Accession # P23510
C-term
中文名
重组人 OX40 配体/TNFSF4 蛋白 (HEK293, His)
同用名
Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFSF4; CD252; TXGP1
氨基酸序列

QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL

分子量

20-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OX40 Ligand/TNFSF4 Protein, Human (HEK293, His)
目录号:
HY-P70622
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