1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. OXT 蛋白, Human (P.pastoris, His)

OXT 蛋白, Human (P.pastoris, His)

目录号: HY-P71766
COA 产品使用指南

OXT 或催产素通过与神经素 1 结合并与催产素受体 (OXTR) 相互作用来诱导生理效应。这种相互作用导致子宫和乳腺平滑肌收缩,通过调节 OXTR 活性分别对分娩和母乳喂养至关重要。OXT 蛋白, Human (P.pastoris, His) 是重组的 OXT 蛋白,由 P. pastoris 表达,带有 N-6*His 标签。OXT 蛋白, Human (P.pastoris, His) 全长 94 个氨基酸,分子量约为 ~16 kDa。

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10 μg ¥2000 In-stock
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描述

OXT, or oxytocin, induces physiological effects by binding to neurophysin 1 and interacting with the oxytocin receptor (OXTR). This interaction results in smooth muscle contraction in the uterus and mammary gland, crucial for labor and breastfeeding, respectively, by modulating OXTR activity. OXT Protein, Human (P.pastoris, His) is the recombinant human-derived OXT protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of OXT Protein, Human (P.pastoris, His) is 94 a.a., with molecular weight of ~16 kDa.

研究背景

OXT 或催产素通过与神经素 1 结合并随后与催产素受体 (OXTR) 相互作用来发挥其生理作用。这种相互作用导致子宫和乳腺的平滑肌收缩。催产素通过调节 OXTR 的活性,在分娩期间介导子宫收缩和促进母乳喂养期间乳汁排出方面发挥着至关重要的作用。

种属

Human

表达系统

P. pastoris

标签

N-6*His

蛋白编号

P01178 (32A-125R)

基因 ID
蛋白结构
N-term
6*His
OXT (32A-125R)
Accession # P01178
C-term
同用名
OXT; OT; Oxytocin-neurophysin 1
氨基酸序列

AAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR

分子量

Approximately 16 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4 or 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

OXT Protein, Human (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OXT Protein, Human (P.pastoris, His)
目录号:
HY-P71766
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