P-Selectin 蛋白, Human (Biotinylated, HEK293, His-Avi)

P-selectin, a Ca(2+)-dependent receptor on myeloid cells, crucially binds to sialyl-Lewis X on neutrophils and monocytes, facilitating the interaction of activated endothelial cells or platelets with leukocytes. This engagement, primarily with SELPLG/PSGL1 and PODXL2, is vital for the rapid rolling of leukocytes during early inflammation. P-selectin's interactions with SNX17 further mediate neutrophil adhesion and rolling, dependent on the sialyl-Lewis X epitope and specific tyrosine sulfation on SELPLG. P-Selectin Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived P-Selectin protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of P-Selectin Protein, Human (Biotinylated, HEK293, His-Avi) is 730 a.a., with molecular weight of 115-140 kDa.

P-选择素是骨髓细胞上的一种 Ca(2+) 依赖性受体，有助于与中性粒细胞和单核细胞上的碳水化合物结合，在活化的内皮细胞或血小板与白细胞的相互作用中发挥着至关重要的作用。P-选择素识别的配体是唾液酸-Lewis X，这种相互作用对于炎症初始阶段白细胞在血管表面快速滚动至关重要，这是通过与 SELPLG 的结合来实现的。此外，P-选择素与 SNX17 形成相互作用，并通过与 SELPLG/PSGL1 和 PODXL2 的结合介导中性粒细胞的粘附和滚动。这种相互作用取决于 SELPLG 和 PODXL2 的唾液酸-Lewis X 表位，以及 SELPLG 上的特定酪氨酸硫酸化。

Measured by its binding ability in a functional ELISA. When immobilized Anti-P-selectin Antibody, hFc Tag at 0.5mg/ml (100μl/Well), can bind Biotinylated Human P-Selectin, His Tag and the EC₅₀ is 0.13 μg/mL.

Human

HEK293

C-Avi;C-His

P16109 (W42-A771)

6403  [NCBI]

115-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.