p24 蛋白, HIV-1 (AAB50258, HEK293, His)

p24 Protein, HIV-1 (AAB50258, HEK293, His) is the recombinant virus-derived p24 protein, expressed by HEK293, with C-His tag.

Gag- pol 多蛋白在病毒粒子组装过程中发挥核心作用，与 Gag 多蛋白合作协调基本事件，如与质膜结合，促进对球形颗粒形成至关重要的蛋白质-蛋白质相互作用，招募病毒 Env 蛋白，以及通过与 RNA包装序列 (Psi) 的直接相互作用包装基因组 RNA。衣壳蛋白 p24 在病毒粒子中形成包封基因组 RNA -核衣壳复合物的锥形核心。大多数核心是圆锥形的，只有 7% 是管状的。核心由衣壳蛋白六聚体亚基组成。核心在病毒粒子进入后很快被分解。衣壳通过隐藏 CGAS 检测的病毒 DNA 来促进免疫入侵。宿主限制因子如 TRIM5-α 或 TRIMCyp 结合逆转录病毒衣壳并导致衣壳过早解体，导致逆转录阻滞。TRIM5 衣壳限制是限制 HIV-1 进入人类的因素之一。宿主 PIN1 明显促进病毒粒子脱壳。另一方面，与 PDZD8 或 CYPA 的相互作用稳定衣壳。

Virus

HEK293

C-His

P04591-1/AAB50258 (P133-L363)

155030

Approximately 26-27 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.