PAK1 蛋白, Human (His-SUMO)

PAK1 is a dynamic protein kinase that plays an important role in multiple intracellular signaling pathways, affecting cytoskeletal dynamics, adhesion, migration, proliferation, apoptosis, mitosis, and vesicle trafficking. Multifunctional PAK1 directly phosphorylates BAD to provide antiapoptotic cell protection. PAK1 Protein, Human (His-SUMO) is the recombinant human-derived PAK1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of PAK1 Protein, Human (His-SUMO) is 545 a.a., with molecular weight of ~76.6 kDa.

PAK1 是一种动态蛋白激酶，复杂地参与整合素和受体型激酶下游的多种细胞内信号传导通路。其多方面的作用包括在细胞骨架动力学、细胞粘附、迁移、增殖、凋亡、有丝分裂和囊泡介导的运输过程中发挥重要作用。PAK1 具有多功能性，可直接磷酸化 BAD，提供针对细胞凋亡的细胞保护。通过与 CDC42 和 RAC1 相互作用实现激活，确立其作为 GTPase 效应子的地位，将 Rho 相关 GTPase 与 JNK MAP 激酶通路连接起来。PAK1 的作用范围延伸至 MAP2K1 的磷酸化和激活，协调下游 MAP 激酶的激活。除了指导肌动蛋白细胞骨架、肌动蛋白应力纤维和粘着斑复合物的重组外，PAK1 还影响微管蛋白伴侣 TBCB，从而影响微管的生物发生和组织。值得注意的作用包括响应血糖水平升高调节胰岛素分泌、参与神经肌肉接头形成以及抑制细胞凋亡期间的活性。PAK1 的广泛功能延伸到磷酸化 RAF1、SNAI1、MYL9/MLC2，并有助于各种细胞功能，例如趋化因子摄取、突触稳定性、树突棘形成和微管成核。它参与介导胃癌细胞迁移、对 DNA 损伤的反应以及促进应激颗粒，强调了 PAK1 细胞影响的复杂性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q13153 (M1-H545)

5058  [NCBI]

MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH

Approximately 76.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.