PAR2 Protein-VLP, Human (HEK293)

PAR2 Protein-VLP, Human (HEK293) is the recombinant human-derived PAR2 protein, expressed by HEK293, with tag free tag.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

PAR2 是一种与 G 蛋白偶联的胰蛋白酶及类胰蛋白酶受体，通过激活多种信号通路（如 phospholipase C (PLC)、细胞内钙、mitogen-activated蛋白激酶(MAPK)、I-kappaB kinase/NF-kappaB 和 Rho）发挥功能。它可被切割的 F2R/PAR1 反式激活，参与调节炎症反应、先天和适应性免疫，并作为感染过程中产生的蛋白水解酶的传感器，通常促进炎症。PAR2 能与 TLR4 和可能 TLR2 协同作用增强炎症反应，并调节 TLR3 信号。在建立内皮屏障中具有保护作用，涉及凝血因子 X；同时调节中性粒细胞外渗期间的内皮细胞屏障完整性（可能通过 PRTN3 蛋白水解切割实现）。在气道上皮中既有支气管保护作用，也可能通过中断 E-cadherin 粘附破坏上皮屏障。参与血管张力调节，激活后通过血管舒张导致低血压。PAR2 与 GNAQ、GNA11 等 G 蛋白 α 亚基结合，但不与 G(o)-α 或特定 G(i) 亚基结合（尽管有研究报道可通过 G(i) α 亚基信号传导）。作为 B 类受体，它通常与 arrestin 形成复合体内化并转运至内体囊泡。通过 GNAQ/GNA11 偶联抑制 TNF-α 刺激的 JNK 磷酸化，并介导 NF-kappa-B RELA 亚基的 Ser-536 磷酸化（该功能主要不依赖 G 蛋白）。此外，PAR2 通过 β-arrestin 支架促进细胞迁移、细胞骨架重组和趋化作用，并参与炎症部位白细胞招募及树突状细胞活化。在先天免疫中增强细菌吞噬和抗病毒反应，与多种急慢性炎症疾病及自身免疫紊乱相关。类似功能（By similarity）：推测介导凝血因子 Xa (F10) 触发的成纤维细胞促炎/促纤维化反应；已证实功能（PubMed:22409427）：介导凝血因子 Xa 触发的内皮细胞屏障保护性信号响应。

Human

HEK293

Tag Free

P55085 (S37-Y397)

2150

溶液.

Supplied as 0.22 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.