PARP 蛋白, Human (sf9, His)

The PARP1 protein is a polyADP-ribosyltransferase that crucially mediates polyADP-ribosylation (PARsylation) in DNA repair, including base excision and double-strand breaks. By interacting with factors such as HPF1 and NMNAT1, the specificity of PARP1 extends to various amino acids. PARP Protein, Human (sf9, His) is the recombinant human-derived PARP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of PARP Protein, Human (sf9, His) is 1014 a.a., with molecular weight of 100-110 kDa.

PARP1 是一种聚 ADP 核糖基转移酶，是蛋白质聚 ADP 核糖基化 (PARsylation) 的重要介质，在 DNA 修复（包括碱基切除修复和双链断裂修复）中发挥着关键作用。HPF1 和 NMNAT1 等相互作用的因子赋予了各种氨基酸的特异性。在 DNA 损伤的情况下，PARP1 识别并结合 DNA 断裂，招募 HPF1 并启动靶蛋白（如组蛋白）的丝氨酸 ADP 核糖基化。这种 PARsylation 促进染色质解压缩、修复因子的募集，并最终修复 DNA 链断裂。此外，PARP1 还参与转录调控，充当转录延伸的正调节因子和负调节因子。它还有助于复制叉进展、parthanatos、cGAS-STING 途径的负调节和脂肪生成抑制等过程。此外，PARP1 与 NMNAT1、PARG 和 NUDT5 合作产生核 ATP，促进广泛的染色质重塑。在细胞凋亡情况下，切割和易位的 PARP1 作为聚 ADP 核糖载体，诱导 AIFM1 介导的细胞凋亡。PARP1 的多方面功能凸显了其在多种细胞过程中不可或缺的作用。

Measured by its binding ability in a functional ELISA. Immobilized human PARP-His at 2 μg/mL (100 μL/well) can bind human HSP70-His and the EC₅₀ is 0.2-1.0 μg/mL.

Human

Sf9 insect cells

C-His

P09874 (M1-W1014)

142  [NCBI]

100-110 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.