PARP3 蛋白, Human (His, StrepⅡ)

The PARP3 protein is a mono-ADP ribosyltransferase that responds to DNA damage, focusing on mono-ADP ribosylation of proteins such as histone H2B, XRCC5, and XRCC6. PARP3 plays a key role in the DNA repair process, participating in precise nonhomologous end joining and inhibiting G-quadruplex structure. PARP3 Protein, Human (His, Strep) is the recombinant human-derived PARP3 protein, expressed by E. coli , with N-His, N-Strep II labeled tag.

PARP3 蛋白是一种单 ADP 核糖基转移酶，在细胞对 DNA 损伤的反应中发挥着关键作用，PubMed 中报道的各种研究都强调了这一点。与 PARP1 和 PARP2 不同，PARP3 特异性介导单 ADP 核糖基化，靶向参与染色质结构和 DNA 代谢的特定蛋白质（例如组蛋白 H2B、XRCC5 和 XRCC6）上的谷氨酸、天冬氨酸或赖氨酸残基。这种蛋白质在 DNA 修复过程中发挥着重要作用，参与 DNA 链断裂触发的检测和信号传导途径。在单链断裂修复中，它催化组蛋白 H2B 的单 ADP 核糖基化，与 XRCC5-XRCC6 异二聚体合作促进精确的非同源末端连接 (NHEJ)。PARP3 还抑制 G-四链体结构，并与各种 DNA 修复因子相关，有助于细胞对外源性和内源性 DNA 损伤的反应。此外，它还充当免疫球蛋白类别转换重组的负调节因子，并且能够对 DNA 链断裂末端进行 ADP 核糖基化，展示了其在基因组维护和细胞过程中的多方面参与。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-StrepⅡ;N-His

Q9Y6F1-1 (M1-L533)

10039

Full Length of Isoform-1

Approximately 63.3 KDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.