PC4/SUB1 蛋白, Human (His)

The PC4/SUB1 protein acts as a multifunctional coactivator that cooperates with TAF to promote functional interactions between upstream activators and the general transcription machinery. Its role extends to the potential stability of multiprotein transcription complexes. PC4/SUB1 Protein, Human (His) is the recombinant human-derived PC4/SUB1 protein, expressed by E. coli , with N-His labeled tag. The total length of PC4/SUB1 Protein, Human (His) is 127 a.a., with molecular weight of ~19 kDa.

PC4/SUB1，也称为正辅因子 4，是一种通用共激活因子，在促进上游激活因子和通用转录机制之间的功能相互作用方面发挥着关键作用。它与 TAF（TBP 相关因子）协同发挥作用，并参与基因表达过程中多蛋白转录复合物的稳定。PC4/SUB1 表现出结合单链 DNA 的能力，以及在体外非特异性结合双链 DNA (dsDNA) 的能力，表明其具有多种 DNA 结合能力。在结构上，它形成同型二聚体，表明可能增强其功能特性的合作排列。此外，PC4/SUB1 与 CSTF2 相互作用，进一步强调其参与与转录调控相关的多种细胞过程。正在进行的研究可能会揭示更多关于 PC4/SUB1 在基因表达中的具体机制和调节功能的见解。

Human

E. coli

N-His

P53999 (M1-L127)

10923  [NCBI]

MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.