1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  4. PD-1
  5. PD-1 蛋白, Human (CHO, Fc)

PD-1 蛋白是一种重要的免疫调节分子,表达于活化的免疫细胞表面。PD-1 蛋白通过与配体 (PD-L1/PD-L2) 结合,发挥免疫抑制的作用。PD-1 蛋白可用于肿瘤和自身免疫性疾病等的研究。PD-1 蛋白, Human (CHO, Fc) 是重组的 PD-1 蛋白,由 CHO 表达,带有 C-hFc 标签。

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5 μg ¥530 In-stock
10 μg ¥850 In-stock
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1 mg ¥17500 In-stock
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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PD-1 Protein is an important immune regulatory molecule expressed on the surface of activated immune cells. By binding to its ligands (PD-L1/PD-L2), the PD-1 Protein exerts immunosuppressive effects. PD-1 Protein can be used in the research of tumors, autoimmune diseases, and other conditions. PD-1 protein, Human (CHO, Fc) is a recombinant PD-1 protein expressed by CHO cells and carries a C-hFc tag[1][2].

研究背景

PD-1 又名 CD279,是一种 55 kDa 的跨膜蛋白,含 288 个氨基酸,包含信号肽、胞外结构域、跨膜结构域和胞内结构域。PD-1 是一种免疫检查点分子,主要表达于活化的 T 细胞、B 细胞等免疫细胞表面。值得注意的是,PD-1 在肿瘤特异性 T 细胞上高度表达。PD-1 是适应性和先天免疫反应的抑制剂。正常情况下,PD-1 通过与配体 PD-L1/PD-L2 结合维持免疫耐受以避免自身免疫损伤。然而,当 PD-1 与肿瘤细胞上的 PD-L1/PD-L2 结合会导致 T 细胞功能耗竭、抑制细胞因子分泌、助力肿瘤免疫逃逸。目前 PD-1 作为肿瘤免疫治疗的核心靶点,其抑制剂通过阻断结合可恢复 T 细胞对肿瘤的杀伤能力[1][2]

体外研究
(In Vitro)

Momordin Ic (HY-N0330) 可阻断 PD-1 (Human) 与 PD-L1 结合[3]

生物活性

1.1 µg/mL (100 µL/well) of immoblized human PD-L1-Fc can bind human Biotin-PD-1-Fc and the ED50 is <25 μg/mL.
2.Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 (HY-P73361) is present at 0.1 μg/mL, can bind Recombinant Human PD-L1. The ED50 for this effect is 0.3479 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 (HY-P73361) is present at 0.1 μg/mL, can bind Recombinant Human PD-L1. The ED50 for this effect is 0.3479 μg/mL.
种属

Human

表达系统

CHO

标签

C-hFc

蛋白编号

Q15116 (L25-Q167)

基因 ID
蛋白结构
N-term
PD-1 (L25-Q167)
Accession # Q15116
hFc
C-term
蛋白长度

Extracellular Domain

中文名
PD-1 蛋白
同用名
rHuPD-1, Fc Chimera; PD1; CD279; PDCD1
氨基酸序列

LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

分子量

Approximately 50-76 kDa due to the glycosylation.

糖基化修饰
Yes
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS or PBS, pH 8.0, 8% trehalose.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PD-1 Protein, Human (CHO, Fc)
目录号:
HY-P7395
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