PD-1 蛋白, Human (CHO, Fc)

PD-1 Protein is an important immune regulatory molecule expressed on the surface of activated immune cells. By binding to its ligands (PD-L1/PD-L2), the PD-1 Protein exerts immunosuppressive effects. PD-1 Protein can be used in the research of tumors, autoimmune diseases, and other conditions. PD-1 protein, Human (CHO, Fc) is a recombinant PD-1 protein expressed by CHO cells and carries a C-hFc tag^[1][2].

PD-1 又名 CD279，是一种 55 kDa 的跨膜蛋白，含 288 个氨基酸，包含信号肽、胞外结构域、跨膜结构域和胞内结构域。PD-1 是一种免疫检查点分子，主要表达于活化的 T 细胞、B 细胞等免疫细胞表面。值得注意的是，PD-1 在肿瘤特异性 T 细胞上高度表达。PD-1 是适应性和先天免疫反应的抑制剂。正常情况下，PD-1 通过与配体 PD-L1/PD-L2 结合维持免疫耐受以避免自身免疫损伤。然而，当 PD-1 与肿瘤细胞上的 PD-L1/PD-L2 结合会导致 T 细胞功能耗竭、抑制细胞因子分泌、助力肿瘤免疫逃逸。目前 PD-1 作为肿瘤免疫治疗的核心靶点，其抑制剂通过阻断结合可恢复 T 细胞对肿瘤的杀伤能力^[1][2]。

Momordin Ic (HY-N0330) 可阻断 PD-1 (Human) 与 PD-L1 结合^[3]。

1.1 µg/mL (100 µL/well) of immoblized human PD-L1-Fc can bind human Biotin-PD-1-Fc and the ED₅₀ is <25 μg/mL.
2.Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 (HY-P73361) is present at 0.1 μg/mL, can bind Recombinant Human PD-L1. The ED₅₀ for this effect is 0.3479 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 (HY-P73361) is present at 0.1 μg/mL, can bind Recombinant Human PD-L1. The ED₅₀ for this effect is 0.3479 μg/mL.

Human

CHO

C-hFc

Q15116 (L25-Q167)

5133  [NCBI]

Extracellular Domain

LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

Approximately 50-76 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS or PBS, pH 8.0, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Syn NL, et al. De-novo and acquired resistance to immune checkpoint targeting. Lancet Oncol. 2017 Dec;18(12):e731-e741.  [Content Brief]

  [2]. Han Y, et al. PD-1/PD-L1 pathway: current researches in cancer. Am J Cancer Res. 2020 Mar 1;10(3):727-742.  [Content Brief]

  [3]. Wu S, et al. Discovery of Momordin Ic that selectively reduces PD-L1 expression in multiple myeloma cells by recruiting SYVN1. Food Bioscience, 2024, 61: 104732.