PD-L2 蛋白, Human (HEK293, Fc)

PD-L2 Protein, Human (HEK293, Fc), a second ligand for PD-1, interacts with PD-1 negatively regulates T cell proliferation, cytokine production and cytotoxic activity.

程序性死亡 I (PD-I) 缺陷小鼠会出现多种自身免疫样疾病，这表明这种免疫抑制受体在耐受性中发挥着重要作用。PD-1 配体 2 (PD-L2) 是 PD-1 的第二个配体。PD-L2 与 PD-1 的结合可显着抑制 T 细胞受体 (TCR) 介导的增殖和 CD4+ T 细胞的细胞因子产生。在低抗原浓度下，PD-L2-PD-1 相互作用会抑制强 B7-CD28 信号。相反，在高抗原浓度下，PD-L2-PD-1 相互作用会减少细胞因子的产生，但不会抑制 T 细胞增殖。PD-L-PD-1 相互作用导致细胞周期停滞在 G0/G1，但不会增加细胞死亡。此外，与单独的 TCR 连接相比，PD-1 + TCR 连接会导致 SHP-2 快速磷酸化^[1]。

5 µg/mL (100 µL/well) of immoblized recombinant human PD-L2-Fc can bind human Biotin-PD-1-Fc with a linear range of 2.44-39.06 ng/mL.

Human

HEK293

C-hFc

Q9BQ51 (L20-P219)

80380  [NCBI]

LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHP

Approximately 75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Latchman Y, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001 Mar;2(3):261-8.  [Content Brief]