PDE3A 蛋白, Human (sf9, His-GST)

PDE3A protein, a cyclic nucleotide phosphodiesterase, targets cAMP, cGMP, and cUMP, crucial in diverse physiological processes. In an E2/17beta-estradiol-induced pro-apoptotic pathway, E2 stabilizes the PDE3A/SLFN12 complex, leading to SLFN12 dephosphorylation and activating its pro-apoptotic ribonuclease activity. This pathway is implicated in tissues with high E2 concentrations, potentially contributing to processes like placenta remodeling. PDE3A Protein, Human (sf9, His-GST) is the recombinant human-derived PDE3A protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PDE3A Protein, Human (sf9, His-GST) is 473 a.a., with molecular weight of ~93 kDa.

PDE3A 蛋白是一种环核苷酸磷酸二酯酶，对第二信使 cAMP 和 cGMP 表现出特异性，而 cAMP 和 cGMP 是多种生理过程中的关键调节因子。此外，PDE3A 还表现出对 cUMP 的活性。除了其催化作用之外，PDE3A 也是 E2/17β-雌二醇诱导的促凋亡信号通路的一个组成部分。雌二醇 (E2) 可稳定细胞质中的 PDE3A/SLFN12 复合物，导致 SLFN12 去磷酸化并激活其促凋亡核糖核酸酶活性。这种细胞凋亡途径与高浓度 E2 的组织有关，并且可能在胎盘重塑等过程中发挥作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

Q14432 (K669-Q1141)

5139  [NCBI]

Approximately 93 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly, 3 mM DTT.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.