1. 重组蛋白
  2. Cytokines and Growth Factors
  3. PDGFs & PDGFRs
  4. PDGF
  5. PDGF-B
  6. PDGF-BB 蛋白, Human (P.pastoris, His)

PDGF-BB 蛋白, Human (P.pastoris, His)

目录号: HY-P73351
COA 产品使用指南

PDGF-BB 蛋白是一种关键的生长因子,集中调节胚胎发育、细胞增殖、迁移、存活和趋化性。它以其对间充质细胞的有效促有丝分裂作用而闻名,对于中枢神经系统、皮肤、肺、心脏和胎盘等组织中周细胞和血管平滑肌细胞的正常增殖和募集至关重要。PDGF-BB 蛋白, Human (P.pastoris, His) 是重组的 PDGF-BB 蛋白,由 P. pastoris 表达,带有 N-His 标签。PDGF-BB 蛋白, Human (P.pastoris, His) 全长 109 个氨基酸,分子量约为 ~14.3 kDa。

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规格 价格 是否有货 数量
50 μg ¥3800 In-stock
100 μg   询价  

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描述

The PDGF-BB protein is a key growth factor that centrally regulates embryonic development, cell proliferation, migration, survival, and chemotaxis. It is known for its potent mitogenic effects on mesenchymal cells and is essential for the normal proliferation and recruitment of pericytes and vascular smooth muscle cells in tissues such as the central nervous system, skin, lungs, heart, and placenta. PDGF-BB Protein, Human (P.pastoris, His) is the recombinant human-derived PDGF-BB protein, expressed by P. pastoris , with N-His labeled tag. The total length of PDGF-BB Protein, Human (P.pastoris, His) is 109 a.a., with molecular weight of ~14.3 kDa.

研究背景

GMP PDGF-BB 蛋白是一种关键的生长因子,在调节胚胎发育、细胞增殖、迁移、存活和趋化性方面发挥着核心作用。GMP PDGF-BB 以其对间充质细胞的强有丝分裂作用而闻名,对于各种组织(包括中枢神经系统、皮肤、肺、心脏和胎盘)中周细胞和血管平滑肌细胞的正常增殖和募集是不可或缺的。它的重要贡献延伸到血管和肾小球的发育,突出了它在血管和肾脏生理学中的重要性。GMP PDGF-BB 是伤口愈合的关键参与者,其信号动力学通过与 PDGFA 形成异二聚体进行微调。GMP PDGF-BB 作为反平行同二聚体存在,与 PDGFRA 和 PDGFRB 同二聚体以及由 PDGFRA 和 PDGFRB 形成的异二聚体进行二硫键连接的相互作用。此外,它还与 PDGFA 形成反向平行异二聚体,进一步使其调控手段多样化。值得注意的是,GMP PDGF-BB 与 XLKD1、LRP1 和 SORL1 建立连接,有助于形成调节其多方面功能的相互作用网络。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-B His at 2 μg/mL (100 μl/well) can bind Human PDGFRBHis & hFc, the EC50 of Human PDGFRBHis & hFc is 9.0-40.0 ng/mL.

种属

Human

表达系统

P. pastoris

标签

N-His

蛋白编号

P01127 (S82-T190)

基因 ID
蛋白结构
N-term
His
PDGF-BB (S82-T190)
Accession # P01127
C-term
同用名
Platelet-derived growth factor subunit B; PDGF subunit B; PDGF2; PDGFB; SIS
分子量

Approximately 14.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 25% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PDGF-BB Protein, Human (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PDGF-BB Protein, Human (P.pastoris, His)
目录号:
HY-P73351
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