PDGF-BB 蛋白, Rat

PDGF-BB Protein, Rat is a member of PDGF family, which promotes cell proliferation, survival and migration, through binding to the tyrosine kinase PDGF receptor.

血小板衍生生长因子 (PDGF) 是间充质细胞和成骨细胞的有效促细胞分裂剂和化学引诱剂，可刺激血管生成分子，从而在骨再生中发挥重要作用^[1]。血小板衍生生长因子-BB是PDGF家族的成员，通过与酪氨酸激酶PDGF受体结合促进细胞增殖、存活和迁移。PDGF-BB对失血性休克发挥有益作用，这与靶向CX43改善血管反应性和血流动力学密切相关。PDGF-BB 通过与 PDGFR 结合在海绵体平滑肌细胞 (CCSMC) 中发挥作用^[2]。

The ED₅₀ is <2 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >5.0 × 10⁵ units/mg.

• 
  Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is 1.528 ng/mL, corresponding to a specific activity is 6.544×10⁵ units/mg.

Rat

E. coli

Tag Free

Q05028 (S74-T182)

24628  [NCBI]

SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPVFKKATVTLEDHLACKCETVVTPRPVT

Approximately 24.6 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM acetic acid or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Badwelan M, et al. The Efficacy of Recombinant Platelet-Derived Growth Factor on Beta-Tricalcium Phosphate to Regenerate Femoral Critical Sized Segmental Defects: Longitudinal In Vivo Micro-CT Study in a Rat Model. J Invest Surg. 2018 Nov 15:1-13.  [Content Brief]

  [2]. Zhao F, et al. Effect of platelet-derived growth factor-BB on gap junction and connexin43 in rat penile corpus cavernosum smooth muscle cells. Andrologia. 2018 Nov 23:e13200.  [Content Brief]