PDGF R alpha 蛋白, Rat (HEK293, His)

PDGF R alpha protein is a tyrosine protein kinase receptor for PDGFA, PDGFB, and PDGFC that coordinates embryonic development, cell proliferation, survival, and chemotaxis. Their functions vary, promoting or inhibiting cell proliferation and migration depending on the situation. PDGF R alpha Protein, Rat (HEK293, His) is the recombinant rat-derived PDGF R alpha protein, expressed by HEK293 , with C-His labeled tag. The total length of PDGF R alpha Protein, Rat (HEK293, His) is 500 a.a., with molecular weight of 70-110 kDa.

PDGF R α 蛋白是一种酪氨酸蛋白激酶，作为 PDGFA、PDGFB 和 PDGFC 的细胞表面受体，在胚胎发育、细胞增殖、存活和趋化性的协调中发挥着关键作用。其多方面的功能包括根据细胞环境促进和抑制细胞增殖和迁移。PDGF R α 是骨髓间充质干细胞分化不可或缺的一部分，对于正常骨骼发育至关重要，并且对于胚胎发育过程中的头颅闭合至关重要。此外，它对于胃肠道粘膜的发育也是不可缺少的，具有募集间充质细胞和促进肠绒毛正常发育的作用。在伤口愈合过程中，PDGF R α 有助于细胞迁移和趋化性。它参与血小板活化、血小板颗粒分泌激动剂以及凝血酶诱导的血小板聚集，突出了其在止血中的重要性。与其同源配体结合可激活多个信号级联反应，其反应受配体性质和 PDGFRA 与 PDGFRB 之间异二聚体形成的调节。PDGF R α 磷酸化 PIK3R1、PLCG1 和 PTPN11，从而激活多种信号通路，包括 AKT1、HRAS 和 MAP 激酶。此外，它还促进 STAT 家族成员的激活，例如 STAT1、STAT3、STAT5A 和/或 STAT5B。受体信号传导受到蛋白磷酸酶和激活受体的快速内化的严格调节。PDGF R α 的复杂功能强调了其在多种生理过程中的关键作用。

Measured by its ability to inhibit the biological activity of PDGF-AA using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.6932 μg/mL in the presence of 100 ng/mL recombinant human PDGF-AA, corresponding to a specific activity is 1.443×10³ U/mg.

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  Measured by its ability to inhibit the biological activity of PDGF-AA using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.6932 μg/mL in the presence of 100 ng/mL recombinant human PDGF-AA, corresponding to a specific activity is 1.443×10³U/mg.

Rat

HEK293

C-His

P20786 (L24-E523)

25267  [NCBI]

LLLPSILPNENEKIVPLSSSFSLRCFGESEVSWQHPMSEEEDPNVEIRTEENNSSLFVTVLEVVNASAAHTGWYTCYYNHTQTEESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIVEEDDSAIIPCLTTDPDTEVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVRGRTFKTSEFNVYALKATSELNLEMDTRQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKATVKDSGDYECAARQATKEVKEMKTVTISVHEKGFVQIRPTFGHLETVNLHQVREFVVEVQAYPTPRISWLKDNLTLIENLTEITTDVQRSQETRYQSKLKLIRAKEEDSGHYTIIVQNDDDMKSYTFELSTLVPASILELVDDHHGSGGGQTVRCTAEGTPLPNIEWMICKDIKKCNNDTSWTVLASNVSNIITEFHQRGRSTVEGRVSFAKVEETIAVRCLAKNDLGIGNRELKLVAPSLRSE

Approximately 70-115 kDa due to glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.