1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. Platelet Factor 4
  6. PF-4/CXCL4 蛋白, Human (HEK293)

PF-4/CXCL4 蛋白, Human (HEK293)

目录号: HY-P7281
COA 产品使用指南

PF-4/CXCL4 是 CXC 趋化因子家族的成员,从活化血小板的 α 颗粒中释放出来。PF-4/CXCL4 以高亲和力结合肝素,具有抗肝素、抗血管生成和免疫调节活性。PF-4/CXCL4 可促进单核细胞存活和巨噬细胞活化。PF-4/CXCL4 蛋白, Human (HEK293) 是在 HEK293 细胞中表达产生的,由 70 个氨基酸(E32-S101) 组成。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥3900 In-stock
100 μg   询价  
250 μg   询价  

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描述

PF-4/CXCL4 is a member of the CXC chemokine family that is released from the alpha-granules of activated platelets. PF-4/CXCL4 binds with high affinity to heparin, with antiheparin, antiangiogenic and immunomodulatory activities. PF-4/CXCL4 plays a role in hematopoiesis and immune cell modulation[1][2]. PF-4/CXCL4 Protein, Human (HEK293) is produced in HEK293 cells, and consists of 70 amino acids (E32-S101).

研究背景

CXCL4, also known as PF-4 (platelet factor-4), is a member of the CXC chemokine family produced by cells of the megakaryocytic lineage.  In megakaryocytes CXCL4 is synthesized, enclosed in vesicles, and transferred to the α granules from which it is secreted following platelet activation. CXCL4 expression is also found in microglia, monocytes and activated T-cells[1][2].
Mature human CXCL4 shares 70% amino acid sequence identity with mouse and rat CXCL4.
CXCL4 is stored in secretory granules of blood platelets and is released in response to protein kinase C and Rac1 activation. Platelets are considered to be the major cellular source of CXCL4. In the α-granules of blood platelets CXCL4 is kept as a tetramer bound to two molecules of chondroitin sulphate. CXCL4 does not possess an ELR acid sequence at its amino terminus and therefore does not bind to CXCR1 or CXCR2. CXCL4 moderates the effects of heparin-like molecules on the endothelial cell surface of blood vessels, thereby inhibiting local antithrombin III activity, which results in a procoagulant role of CXCL4. CXCL4 exhibits antiangiogenic properties in vitro and in vivo and inhibits tumor neovascularization through a variety of mechanisms. First, CXCL4 is able to interact directly with angiogenic growth factors, such as FGFs and VEGF, and inhibits their interaction with the cell surface receptor. Second, CXCL4 may bind proteoglycans and interfere with the proteoglycan-bystander effect on growth factor activity. Furthermore, a cell surface receptor that is expressed by human endothelial cells (ECs) in a cell cycle-dependent manner11 and mediates the antiangiogenic effects of CXCL4 has been recently identified and named as CXCR3-B. CXCL4 has been shown to modulate the proliferation, phenotype and function of immune cells. For instance, CXCL4 has been reported to promote monocyte survival and macrophage activation. CXCL4 induces migration of activated T lymphocytes[1][2][3].
CXCL4 shows pleiotropic biological functions. Firstly, CXCL4 activates platelets, modulates platelet aggregation and stimulates release of α-granule proteins. CXCL4 has a role in heparin-induced thrombocytopenia (HIT). Secondly, CXCL4 inhibits endothelial cell proliferation and migration, leading to suppression of angiogenesis. Thirdly, CXCL4 expresses immunomodulatory activities, such as down-regulation of IFN- production by type 1 T-helper (Th1) cells and up-regulation of IL-4, IL-5, and IL-13 in type 2 T-helper (Th2) cells. Fourthly, CXCL4 influences hematopoiesis, inhibiting megakaryocytopoiesis and the proliferation of committed erythroid and granulocyte-macrophage colonies, as well as of primitive CD34+ progenitors. Moreover, CXCL4 is also highly upregulated in plasmacytoid dendritic cells (pDCs) in systemic sclerosis and dendritic cells (DCs) after severe trauma[1][2][3].

体内研究
(In Vivo)

Recombinant human CXCL4 (10 μg/mL; for 6 days) treatment monocytes differentiating into dendritic cells (moDCs) induces a higher IL-17 production by CD4+ T cells. CXCL4 treatment also increases the percentage of IFN-γ-producing cells and the percentage of co-expressing IL17+IL-22+ cells. CXCL4-treated moDCs also significantly potentiates CD4+ T cell proliferation[4].
Recombinant human CXCL4/PF-4 (8 μg/ml) affects the production of cytokines by human Th1 and Th2 cell lines, in particular, it down-regulated the Th1 cytokine IFN-γ and increased the mRNA levels of Th2 cytokines IL-4, IL-5 and IL-13[5].

生物活性

The ED50 is <10 μg/mL as measured by its ability to inhibit human FGF-basic-dependent proliferation of NR6R 3T3 mouse fibroblast cells.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P02776 (E32-S101)

基因 ID

5196  [NCBI]

中文名
重组人 CXC 趋化因子4 (HEK293)
同用名
rHuPF-4/CXCL4; C-X-C motif chemokine 4; Oncostatin A; SCYB4
氨基酸序列

EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

分子量

Approximately 7.8 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PF-4/CXCL4 Protein, Human (HEK293)
目录号:
HY-P7281
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