PFDN5 蛋白, Human (His)

PFDN5 protein selectively binds to cytosolic chaperonin (c-CPN), facilitating targeted protein transfer and promoting nascent polypeptide folding. Beyond its chaperone function, PFDN5 regulates MYC transcriptional activity by forming a heterohexamer. It interacts with MYC's N-terminal domain, showcasing a multifaceted role in cellular processes, bridging protein folding and transcriptional regulation. PFDN5 Protein, Human (His) is the recombinant human-derived PFDN5 protein, expressed by E. coli, with N-His labeled tag. The total length of PFDN5 Protein, Human (His) is 154 a.a., with molecular weight of ~20 kDa.

PFDN5 蛋白表现出与胞质伴侣蛋白 (c-CPN) 的靶向结合，促进特定蛋白质向该伴侣的转移。此外，PFDN5 与新生多肽链结合，积极支持它们在充满非天然蛋白质竞争途径的细胞环境中正确折叠。除了其伴侣功能之外，PFDN5 还通过抑制 MYC 的转录活性发挥调节作用。在结构上，PFDN5形成包含两个PFD-α型亚基和四个PFD-β型亚基的异六聚体。值得注意的是，它与 MYC 的 N 末端结构域发生特异性相互作用，表明 PFDN5 在细胞过程中发挥多方面的作用，包括蛋白质折叠和转录调节。

Human

E. coli

N-His

Q99471 (M1-A154)

5204  [NCBI]

MAQSINITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA

Approximately 20 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.