2. 重组蛋白
  3. Enzymes & Regulators
  4. Isomerases (EC 5)
  5. PGAM1 蛋白, Human (His, Strep)

PGAM1 蛋白, Human (His, Strep)

目录号: HY-P701963
产品使用指南

PGAM1 蛋白通过催化 2-磷酸甘油酸和 3-磷酸甘油酸的重要相互转化，在糖酵解中发挥关键作用。此外，它有助于将 (2R)-2,3-二磷酸甘油酸转化为 (2R)-3-磷酸甘油酰磷酸，进一步促进与糖酵解途径相关的复杂代谢过程。PGAM1 蛋白, Human (His, Strep) 是重组的 PGAM1 蛋白，由 E. coli 表达，带有 N-Strep, N-6*His 标签。PGAM1 蛋白, Human (His, Strep) 全长 254 个氨基酸。

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描述

PGAM1 protein plays a pivotal role in glycolysis by catalyzing the vital interconversion of 2-phosphoglycerate and 3-phosphoglycerate. Additionally, it is instrumental in the conversion of (2R)-2,3-bisphosphoglycerate to (2R)-3-phospho-glyceroyl phosphate, further contributing to the intricate metabolic processes associated with glycolytic pathways. PGAM1 Protein, Human (His, Strep) is the recombinant human-derived PGAM1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of PGAM1 Protein, Human (His, Strep) is 254 a.a., .
研究背景

PGAM1（或磷酸甘油酸变位酶 1）通过催化 2-磷酸甘油酸和 3-磷酸甘油酸的可逆相互转化，在糖酵解中发挥关键作用。这种酶活性是糖酵解途径中的关键步骤，促进葡萄糖衍生的底物转化为能量和代谢中间体。此外，PGAM1 还参与 (2R)-2,3-二磷酸甘油酸酯和 (2R)-3-磷酸甘油酰磷酸的相互转化。这些反应的调节对于维持糖酵解通量和确保代谢物通过糖酵解途径的适当流动至关重要。PGAM1 的酶功能有助于细胞内能量产生和代谢稳态的整体效率。
种属

Human
表达系统

E. coli
标签

N-Strep;N-6*His
蛋白编号

P18669 (M1-K254)
基因 ID

5223
蛋白结构
N-term
Strep-6*His
PGAM1 (M1-K254)
Accession # P18669
C-term
同用名
PGAM1; Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PGAM1 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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