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  3. PGD 蛋白, Human (His)

PGD 蛋白, Human (His)

目录号: HY-P73376
产品使用指南

PGD 蛋白在细胞代谢中至关重要,可催化 6-磷酸葡萄糖酸的氧化脱羧。该过程产生 5-磷酸核酮糖,释放 CO(2),同时将 NADP 还原为 NADPH,有助于维持重要的细胞氧化还原平衡。该酶的功能强调了其在维持细胞稳态和参与关键代谢途径方面的重要性。PGD 蛋白, Human (His) 是重组的 PGD 蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

PGD Protein is crucial in cellular metabolism, catalyzing the oxidative decarboxylation of 6-phosphogluconate. This process yields ribulose 5-phosphate, releases CO(2), and simultaneously reduces NADP to NADPH, contributing to essential cellular redox balance. The enzyme's functionality underscores its significance in maintaining cellular homeostasis and participating in key metabolic pathways. PGD Protein, Human (His) is the recombinant human-derived PGD protein, expressed by E. coli , with N-His labeled tag.

研究背景

PGD 蛋白通过催化 6-磷酸葡萄糖酸的氧化脱羧,导致形成 5-磷酸核酮糖并释放 CO(2),在细胞代谢中发挥关键作用。该酶促过程与 NADP 同时还原为 NADPH 相结合,有助于维持重要的细胞氧化还原平衡。PGD 蛋白介导这些生化反应的功能强调了其在维持细胞稳态和参与关键代谢途径中的重要性。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P52209-1 (M1-A483)

基因 ID

5226  [NCBI]

蛋白结构
N-term
His
PGD (M1-A483)
Accession # P52209-1
C-term
同用名
6-phosphogluconate dehydrogenase, decarboxylating; PGD; PGDH
分子量

Approximately 46 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PGD Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PGD Protein, Human (His)
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HY-P73376
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