PHYH 蛋白, Human

PHYH protein catalyzes 2-hydroxylation of various mono-branched and straight-chain acyl-CoA esters, including racemic phytanoyl-CoA and isomers of 3-methylhexadecanoyl-CoA. It acts on acyl-CoAs with a chain length of at least seven carbon atoms, excluding long, very long straight-chain, and 2-methyl or 4-methyl-branched acyl-CoAs. PHYH Protein, Human is the recombinant human-derived PHYH protein, expressed by E. coli , with tag free.

PHYH 蛋白通过催化各种酰基辅酶 A 酯（包括外消旋植酰基辅酶 A、3-甲基十六酰辅酶 A 的异构体以及多种其他单支链 3-甲基酰基辅酶 A）的 2-羟基化，在细胞代谢中发挥着至关重要的作用链长至少为 7 个碳原子的酯，以及链长超过 4 个碳原子的直链酰基辅酶 A 酯。值得注意的是，PHYH 不会羟基化长链和超长直链酰基辅酶A或2-甲基和4-甲基支链酰基辅酶A，显示出其底物特异性。这些酶活性有助于脂质代谢和细胞稳态的调节，凸显了 PHYH 在维持代谢平衡中的重要性。

Measured by its ability to catalyze a reaction to produce inorganic phosphates at 37°C for 30 min. The specific activity is 1104.22 pmol/min/mg.

Human

E. coli

Tag Free

O14832-1 (S31-L338)

5264  [NCBI]

Full Length of Mature Protein

SGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.