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  3. PIK3IP1 蛋白, Mouse (HEK293, His)

PIK3IP1 蛋白, Mouse (HEK293, His)

目录号: HY-P77139
COA 产品使用指南

PIK3IP1 蛋白与磷脂酰肌醇 3-激酶的催化亚基结合。它对磷脂酰肌醇 3-激酶活性和信号传导产生负调节。PIK3IP1 蛋白, Mouse (HEK293, His) 是重组的 PIK3IP1 蛋白,由 HEK293 表达,带有 C-His 标签。PIK3IP1 蛋白, Mouse (HEK293, His) 全长 149 个氨基酸,分子量约为 ~23 KDa。

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5 μg ¥500 In-stock
10 μg ¥850 In-stock
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100 μg ¥4000 In-stock
500 μg ¥12000 In-stock
1 mg ¥20400 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The PIK3IP1 protein binds to the catalytic subunit of phosphatidylinositol 3-kinase. It negatively regulates phosphatidylinositol 3-kinase activity and signaling. PIK3IP1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PIK3IP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PIK3IP1 Protein, Mouse (HEK293, His) is 149 a.a., with molecular weight of ~23 KDa.

研究背景

PIK3IP1 蛋白预计具有磷脂酰肌醇 3-激酶催化亚基结合活性。它在磷脂酰肌醇 3-激酶活性和磷脂酰肌醇 3-激酶信号传导的负调节中发挥作用。预计该蛋白质位于质膜中并作为膜的组成部分。它在多种结构中表达,包括脑膜、泌尿生殖系统、心脏、肺和口腔上皮。它与人类 PIK3IP1(磷酸肌醇 3 激酶相互作用蛋白 1)是直系同源的。在表达方面,它在成体胸腺、成体睾丸和24种其他组织中广泛表达。

生物活性

Data is not available.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

NP_835362.2 (S22-L170)

基因 ID

216505  [NCBI]

蛋白结构
N-term
PIK3IP1 (S22-L170)
Accession # NP_835362.2
His
C-term
同用名
Kringle domain-containing protein HGFL; PIK3IP1; HGFL
氨基酸序列

SGGCFWDNGHLYREDQPSPAPGLRCLNWLAAQGSRESLTEPSPGNHNYCRNPDQDPRGPWCYISSETGVPEKRPCEDVSCPETTSQAPPPSSAMELEEKSGAPGDKEAQVFPPANALPARSEAAEVQPVIGISQLVRMNSKEKKDLGTL

分子量

Approximately 23 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PIK3IP1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PIK3IP1 Protein, Mouse (HEK293, His)
目录号:
HY-P77139
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