PIM1 蛋白, Human

The PIM1 protein is a proto-oncogene with serine/threonine kinase activity that significantly promotes cell survival and proliferation, providing a selective advantage for tumorigenesis. It regulates MYC transcription, cell cycle progression, and inhibits pro-apoptotic proteins such as BAD, MAP3K5, and FOXO3 through phosphorylation. PIM1 Protein, Human is the recombinant human-derived PIM1 protein, expressed by E. coli , with tag free.

PIM1蛋白是一种具有丝氨酸/苏氨酸激酶活性的原癌基因，在细胞存活和增殖中发挥着关键作用，在肿瘤发生中具有选择性优势。其致癌活性涉及调节 MYC 转录活性、细胞周期进程以及磷酸化介导的促凋亡蛋白（包括 BAD、MAP3K5 和 FOXO3）的抑制。PIM1 磷酸化 MYC，增强其稳定性和转录活性，这可能解释了 PIM1 和 MYC 在肿瘤发生中的协同作用。通过磷酸化 BAD，PIM1 诱导抗凋亡蛋白 Bcl-X(L)/BCL2L1 的释放，促进生存信号传导。此外，PIM1 磷酸化 MAP3K5，抑制其激酶活性并抑制 MAP3K5 介导的细胞凋亡信号传导。PIM1 通过磷酸化 CDC25A 和 CDC25C 来刺激 G1-S 和 G2-M 转变的细胞周期进程，并在转录和翻译后水平下调细胞周期调节因子 CDKN1B。PIM1 还通过磷酸化 HP1 gamma/CBX3 影响染色质结构，并充当骨髓细胞归巢和迁移的调节剂。此外，PIM1 正向调节 mTORC1 信号传导，通过 ABCG2 磷酸化和激活抑制药物敏感性，并促进棕色脂肪细胞分化。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.

Human

E. coli

Tag Free

P11309 (A14-K313)

5292

Partial

34.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.