1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKACα 蛋白, Human (Sf9, GST)

PKACα 蛋白, Human (Sf9, GST)

目录号: HY-P701753
产品使用指南

PKACα 蛋白是一种关键激酶,可磷酸化多种底物,包括 CDC25B、ABL1、NFKB1 和 VASP,影响多种细胞过程,如细胞周期进程、血小板调节和脂肪形成分化。它还通过 RPTOR 磷酸化对 mTORC1 进行负调节,从而调节信号网络。PKACα 蛋白, Human (Sf9, GST) 是重组的 PKACα 蛋白 (Sf9, GST),由 Sf9 insect cells 表达,带GST标签。PKACα 蛋白, Human (Sf9, GST) 全长 350 个氨基酸。

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描述

PKACα protein is a key kinase that phosphorylates a variety of substrates, including CDC25B, ABL1, NFKB1, and VASP, affecting a variety of cellular processes, such as cell cycle progression, platelet regulation, and adipogenic differentiation. It also negatively regulates mTORC1 through RPTOR phosphorylation, thereby modulating signaling networks. PKACα Protein, Human (Sf9, GST) is the recombinant human-derived PKACα protein, expressed by Sf9 insect cells , with GST tagged. The total length of PKACα Protein, Human (Sf9, GST) is 350 a.a., .

研究背景

PKACα 是一种蛋白激酶,通过磷酸化细胞质和细胞核中的无数底物,在细胞调节中发挥关键作用。其广泛的底物库包括 CDC25B、ABL1、NFKB1、CLDN3、PSMC5/RPT6、PJA2、RYR2、RORA、SOX9 和 VASP 等。通过特定底物的磷酸化,PKACα 影响多种细胞过程,如细胞周期进程、血小板调节、脂肪形成和成骨分化、软骨形成和紧密连接动力学。值得注意的是,PKACα 通过磷酸化 RPTOR 来负向调节 mTORC1,从而充当复杂信号网络中的关键调节剂。此外,PKACα 对胚胎发育、Hedgehog 信号传导、减数分裂恢复,甚至快速眼动睡眠发挥调节作用,展示了其在细胞生理学和体内平衡中的多方面作用。

种属

Human

表达系统

Sf9 insect cells

标签

GST

蛋白编号

P17612 (G2-F351)

基因 ID

5566

同用名
PRKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PKACα Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PKACα Protein, Human (Sf9, GST)
目录号:
HY-P701753
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