1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Kinase Inhibitor Peptide (PKI)
  4. cAMP-Dependent Protein Kinase A Inhibitor beta
  5. PKI-beta 蛋白, Human (His)

PKI-beta 蛋白, Human (His)

目录号: HY-P71209
COA 产品使用指南

PKI-β 蛋白成为 cAMP 依赖性蛋白激酶活性的极其有效的竞争性抑制剂。该蛋白质在分子水平上发挥作用,在 cAMP 诱导其调节链解离后与酶的催化亚基结合。PKI-beta 蛋白, Human (His) 是重组的 PKI-β 蛋白,由 E. coli 表达,带有 N-6*His 标签。PKI-beta 蛋白, Human (His) 全长 78 个氨基酸,分子量约为 ~14.0 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1700 In-stock
50 μg ¥5100 In-stock
100 μg   询价  

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描述

The PKI-β protein emerged as an extremely potent competitive inhibitor of cAMP-dependent protein kinase activity. This protein operates at the molecular level, binding to the catalytic subunit of the enzyme after cAMP induces dissociation of its regulatory chain. PKI-beta Protein, Human (His) is the recombinant human-derived PKI-beta protein, expressed by E. coli , with N-6*His labeled tag. The total length of PKI-beta Protein, Human (His) is 78 a.a., with molecular weight of ~14.0 kDa.

研究背景

PKI-β 蛋白是一种极其有效的 cAMP 依赖性蛋白激酶活性竞争性抑制剂。作为调节分子,PKI-β 通过与酶的催化亚基相互作用来发挥其抑制作用,特别是在 cAMP 诱导其调节链解离后。这种调节机制强调了 cAMP 依赖性蛋白激酶活性的动态性质以及 PKI-β 在调节该信号通路中的关键作用。通过严格调节催化亚基,PKI-β 在调节细胞对 cAMP 信号传导的反应中发挥着至关重要的作用,有助于微调受这种重要激酶影响的细胞内过程,例如与细胞生长、代谢和基因表达相关的过程。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9C010 (M1-K78)

基因 ID
蛋白结构
N-term
6*His
PKI-beta (M1-K78)
Accession # Q9C010
C-term
同用名
cAMP-Dependent Protein Kinase Inhibitor Beta; PKI-beta; PKIB; PRKACN2
氨基酸序列

MRTDSSKMTDVESGVANFASSARAGRRNALPDIQSSAATDGTSDLPLKLEALSVKEDAKEKDEKTTQDQLEKPQNEEK

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PKI-beta Protein, Human (His)
目录号:
HY-P71209
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