1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKM2 蛋白, Human (420 a.a, His)

PKM2 蛋白, Human (420 a.a, His)

目录号: HY-P7803
COA 产品使用指南

PKM2 蛋白, Human (420 a.a, His)是一种多功能蛋白,仅在胚胎和成人分裂/肿瘤细胞中表达。PKM2 可以分解葡萄糖并参与许多非糖酵解途径。

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规格 价格 是否有货 数量
10 μg ¥880 In-stock
50 μg ¥2450 In-stock
100 μg   询价  

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描述

PKM2 Protein, Human (420 a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways[1].

研究背景

丙酮酸激酶 M2 (PKM2) 是一种多功能蛋白,仅在胚胎和成体分裂/肿瘤细胞中表达。PKM2是一种普遍存在的原型酶,在胚胎阶段存在于所有组织中,并在发育过程中逐渐被特定组织中的其他同工酶形式所取代。尽管 PKM2 的主要功能是分解代谢葡萄糖,但它也参与许多非糖酵解途径,影响细胞生理学[1]

生物活性

Measured by its ability to couple reaction catalyzed by PKM2 and LDH, detect the consumption of NADH by pyruvate, the product of phosphate (enol) pyruvate monosodium salt hydrate (PEP). Specific activity is >12.13 pmol/min/μg was measured under the conditions described.

种属

Human

表达系统

E. coli

标签

His

蛋白编号

P14618.4 (L61-E480)

基因 ID
同用名
rHuPyruvate Kinase M2/PKM2, His; Pyruvate Kinase M2; PKM2; CTHBP; OIP-3; THBP1
氨基酸序列

LKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE

分子量

Approximately 55 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filter solution of 50 Mm Tris, 1 mM EDTA, trace Imidazole and DTT, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

PKM2 Protein, Human (420 a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PKM2 Protein, Human (420 a.a, His)
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