1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKM2 蛋白, Human (420 a.a, His)

PKM2 蛋白, Human (420 a.a, His)

目录号: HY-P7803
产品使用指南

PKM2 蛋白, Human (420 a.a, His)是一种多功能蛋白,仅在胚胎和成人分裂/肿瘤细胞中表达。PKM2 可以分解葡萄糖并参与许多非糖酵解途径。

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250 μg ¥6850 询问价格 & 货期

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描述

PKM2 Protein, Human (420 a.a, His) is a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 can catabolize glucose and involved in many nonglycolytic pathways[1].

研究背景

Pyruvate Kinase M2 (PKM2), a multifunctional protein, is exclusively expressed in embryonic and adult dividing/tumor cells. PKM2 is a ubiquitous prototype enzyme present in all tissues during the embryonic stage and is gradually replaced by other isozymic forms in specific tissues during development. Although the primary function of PKM2 is to catabolize glucose, it is involved in many nonglycolytic pathways, influencing the cellular physiology[1].

生物活性

Measured by its ability to transfer phosphate from phospho(enol)pyruvic acid monosodium salt hydrate (PEP) to adenosine 5'-diphosphate sodium salt (ADP). The specific activity is >1000 units/mg, as measured under the described conditions.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P14618.4(L61-E480)

基因 ID
同用名
rHuPyruvate Kinase M2/PKM2, His; Pyruvate Kinase M2; PKM2; CTHBP; OIP-3; THBP1
氨基酸序列

LKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE

分子量

Approximately 55 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

Solution

组分

Supplied as a 0.2 μm filter solution of 50 Mm Tris, 1 mM EDTA, trace Imidazole and DTT, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

PKM2 Protein, Human (420 a.a, His) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PKM2 Protein, Human (420 a.a, His)
目录号:
HY-P7803
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