PLK1 蛋白, Human (sf9, His)

PLK1 is a serine/threonine protein kinase that centrally coordinates key functions during the M phase of the cell cycle. It regulates centrosome maturation, spindle assembly, cohesin removal, inactivates APC/C inhibitors, and controls mitotic exit and cytokinesis. PLK1 Protein, Human (sf9, His) is the recombinant human-derived PLK1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PLK1 Protein, Human (sf9, His) is 603 a.a., with molecular weight of ~66 kDa.

PLK1 是一种丝氨酸/苏氨酸蛋白激酶，在协调整个细胞周期 M 期的各种基本功能中发挥着关键作用。它调节中心体成熟和纺锤体组装，促进从染色体臂去除粘连蛋白，使后期促进复合物/环体 (APC/C) 抑制剂失活，并控制有丝分裂退出和胞质分裂。PLK1 通过结合和磷酸化已在 POLO 盒结构域识别的特定基序上磷酸化的蛋白质进行操作，从而磷酸化多种底物，包括 BORA、BUB1B/BUBR1、CCNB1、CDC25C、CEP55、ECT2、ERCC6L、FBXO5/EMI1、FOXM1 、KIF20A/MKLP2、CENPU、NEDD1、NINL、NPM1、NUDC、PKMYT1/MYT1、PRC1、RACGAP1/CYK4、SGO1、STAG2/SA2、TEX14、TOPORS、p73/TP73、TPT1、WEE1、HNRNPU 等。PLK1 的关键作用包括通过 KIZ、NEDD1 和 NINL 的磷酸化促进中心体功能和双极纺锤体组装。此外，它还通过磷酸化 CEP55、ECT2、KIF20A/MKLP2、CENPU、PRC1 和 RACGAP1 来控制有丝分裂退出和胞质分裂。PLK1 的参与延伸到着丝粒功能、姐妹染色单体凝聚力以及各种检查点蛋白的调节，使其成为控制细胞周期进展的复杂网络中的关键参与者。

The specific activity was determined to be > 3 nmol/min/mg using casein as substrate.

Human

Sf9 insect cells

N-His

P53350 (M1-S603)

5347  [NCBI]

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 0.5 mM PMSF, 25% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.