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PLRP1 蛋白, Human (HEK293, His)

目录号: HY-P70991
产品使用指南

PLRP1 蛋白是由 PNLIPRP1 基因编码的蛋白质。PLRP1 由胰腺腺泡细胞产生并在胰腺中表达。PLRP1 参与脂质分解代谢过程,过与胰腺脂肪酶竞争辅脂肪酶占据,充当胰腺脂肪酶活性的负调节因子。并它可能作为膳食甘油三酯消化的抑制剂。PLRP1 蛋白, Human (HEK293, His) 是重组的 PLRP1 蛋白,由 HEK293 表达,带有 C-6*His 标签。PLRP1 蛋白, Human (HEK293, His) 全长 450 个氨基酸,分子量为 44-50 kDa。

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描述

PLRP1 Protein is a protein that encoded by PNLIPRP1 genne. PLRP1 is produced by pancreatic acinar cells and expressed in the pancreas. PLRP1 is involved in lipid catabolic process, acting as a negative regulator of pancreatic lipase activity by competing with pancreatic lipase for colipase occupancy. And it may function as inhibitor of dietary triglyceride digestion. PLRP1 Protein, Human (HEK293, His) is the recombinant human-derived PLRP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PLRP1 Protein, Human (HEK293, His) is 450 a.a., with molecular weight of 44-50 kDa.

研究背景

胰脂肪酶相关蛋白 1 (PLRP1) 是由 PNLIPRP1 基因编码的蛋白质。PLRP1 由胰腺腺泡细胞产生并在胰腺中表达。PLRP1 参与脂质分解代谢过程,通过与胰腺脂肪酶竞争辅脂肪酶占据,充当胰腺脂肪酶活性的负调节因子。PLRP1 可能在抑制膳食甘油三酯消化中发挥作用,但它对甘油三酯、甘油二酯、磷脂酰胆碱、半乳糖脂或胆固醇酯缺乏可检测到的脂肪酶活性。PLRP1 可以与辅脂肪酶结合,并在辅脂肪酶存在的情况下抑制 PTL 活性。此外,缺乏PLRP1可能会导致肥胖以及葡萄糖不耐受和胰岛素抵抗的症状[1][2][3]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH25784.1 (K18-C467)

基因 ID
蛋白结构
N-term
PLRP1 (K18-C467)
Accession # AAH25784.1
6*His
C-term
同用名
Pancreatic Lipase-Related Protein 1; PL-RP1; PNLIPRP1; PLRP1
氨基酸序列

KEVCYEDLGCFSDTEPWGGTAIRPLKILPWSPEKIGTRFLLYTNENPNNFQILLLSDPSTIEASNFQMDRKTRFIIHGFIDKGDESWVTDMCKKLFEVEEVNCICVDWKKGSQATYTQAANNVRVVGAQVAQMLDILLTEYSYPPSKVHLIGHSLGAHVAGEAGSKTPGLSRITGLDPVEASFESTPEEVRLDPSDADFVDVIHTDAAPLIPFLGFGTNQQMGHLDFFPNGGESMPGCKKNALSQIVDLDGIWAGTRDFVACNHLRSYKYYLESILNPDGFAAYPCTSYKSFESDKCFPCPDQGCPQMGHYADKFAGRTSEEQQKFFLNTGEASNFARWRYGVSITLSGRTATGQIKVALFGNKGNTHQYSIFRGILKPGSTHSYEFDAKLDVGTIDKVKFLWNNNVINPTLPKVGATKITVQKGEEKTVYNFCSEDTVREDTLLTLTPC

分子量

44-50 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PLRP1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PLRP1 Protein, Human (HEK293, His)
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HY-P70991
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