PLY 蛋白, Streptococcus pneumoniae serotype 4 (N-His)

The PLY protein, a cholesterol-dependent toxin, induces cytolysis by forming pores in host membranes with a significant conformational change and oligomeric pore complex formation. Cholesterol is crucial for binding, insertion, and pore formation. PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is the recombinant PLY protein, expressed by E. coli , with N-His labeled tag. The total length of PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is 470 a.a., with molecular weight of ~55 kDa.

PLY 蛋白作为一种胆固醇依赖性毒素，通过在含有胆固醇的宿主膜上形成孔来诱导细胞溶解。与靶膜结合后，蛋白质经历显着的构象变化，促进其插入宿主膜并随后形成寡聚孔复合物。胆固醇的存在对于该过程的各个阶段都至关重要，包括与宿主膜的结合、膜插入和孔形成，其中胆固醇结合由 C 末端的 Thr-Leu 对介导。值得注意的是，PLY 蛋白具有可逆性，可以通过氧化失活。所得同源寡聚孔复合物包含 35 至 50 个亚基，在插入宿主膜后形成，有助于蛋白质的溶细胞活性。

Others

E. coli

N-His

P0C2J9 (A2-D471)

/

ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration, please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.