1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PNKP 蛋白, Human (His-SUMO)

PNKP 蛋白, Human (His-SUMO)

目录号: HY-P71620
COA 产品使用指南

PNKP 蛋白通过 NHEJ 和 BER 途径在 DNA 损伤修复中发挥关键作用。其双重催化活性包括从 DNA 主链上去除 3'-磷酸基团和磷酸化 5'-羟基基团,确保延伸和连接过程中 DNA 末端的兼容性。PNKP 蛋白, Human (His-SUMO) 是重组的 PNKP 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。PNKP 蛋白, Human (His-SUMO) 全长 521 个氨基酸,分子量约为 ~70 kDa。

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规格 价格 是否有货 数量
20 μg ¥1700 In-stock
50 μg ¥3150 In-stock
100 μg ¥5040 询价
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PNKP protein plays a key role in DNA damage repair through NHEJ and BER pathways. Its dual catalytic activity includes removal of the 3'-phosphate group from the DNA backbone and phosphorylation of the 5'-hydroxyl group, ensuring compatibility of DNA ends during extension and ligation. PNKP Protein, Human (His-SUMO) is the recombinant human-derived PNKP protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of PNKP Protein, Human (His-SUMO) is 521 a.a., with molecular weight of ~70 kDa.

研究背景

PNKP 蛋白在 DNA 损伤修复中发挥着至关重要的作用,积极参与非同源末端连接 (NHEJ) 和碱基切除修复 (BER) 途径。利用其两种催化活性,PNKP 在维持 DNA 末端兼容性以进行后续延伸和连接过程中发挥着关键作用。它通过从 DNA 主链上去除 3'-磷酸基团或磷酸化核糖上的 5'-羟基基团来实现这一点。这种双重功能凸显了 PNKP 在解决各种类型的 DNA 损伤、确保基因组的完整性以及致力于 DNA 修复和维护的复杂细胞机制网络方面的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

Q96T60 (1M-521G)

基因 ID
蛋白结构
N-term
6*His-SUMO
PNKP (1M-521G)
Accession # Q96T60
C-term
同用名
2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; Polynucleotide kinase-3''-phosphatase
氨基酸序列

MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

分子量

Approximately 70 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PNKP Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PNKP Protein, Human (His-SUMO)
目录号:
HY-P71620
需求量: