Podoplanin 蛋白, Human (HEK293, His-Fc)

The Podoplanin protein is a multifaceted regulator that affects cell migration and adhesion through multiple interactions. In hemo-lymphatic dissociation, Podoplanin binds to CLEC1B, triggering platelet activation that is counteracted by the CD9 interaction. Podoplanin Protein, Human (HEK293, His-Fc) is the recombinant human-derived Podoplanin protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of Podoplanin Protein, Human (HEK293, His-Fc) is 123 a.a., with molecular weight of ~50.2 kDa.

Podoplanin 蛋白作为多方面的调节剂出现，通过与各种伙伴的相互作用对细胞迁移和粘附产生多种影响。在发育过程中，Podoplanin 通过与 CLEC1B 结合，触发血小板活化和聚集，在血管和淋巴管的分离中发挥着至关重要的作用。相反，它与 CD9 的相互作用减弱了 Podoplanin 诱导的血小板聚集。该蛋白通过与 MSN 或 EZR 相互作用，促进上皮间质转化 (EMT)，从而增加细胞迁移和侵袭性。与 CD44 结合有利于上皮细胞和肿瘤细胞的定向细胞迁移。在淋巴结中，Podoplanin 通过维持 ERM 蛋白（EZR、MSN 和 RDX）和 MYL9 激活来控制成纤维网状细胞 (FRC) 与细胞外基质 (ECM) 的粘附和肌动球蛋白的收缩。与 CLEC1B 的结合通过阻断侧膜相互作用来促进 FRC 松弛。Podoplanin 还通过与 LGALS8 的相互作用参与淋巴内皮与周围 ECM 的连接。在角质形成细胞中，Podoplanin 诱导形态变化、增加运动性并降低细胞粘附。此外，Podoplanin 调节肿瘤细胞中侵袭伪足的稳定性和成熟，有助于 ECM 的有效降解。该蛋白是同二聚体，可与一系列伙伴相互作用，包括 CLEC1B、CD9、LGALS8、CD44、MSN、EZR 和 CCL21，显示其复杂地参与各种细胞过程和信号传导途径。进一步的研究对于揭示 Podoplanin 在这些不同细胞功能中的精确分子机制和更广泛的影响至关重要。

Measured by its binding ability in a functional ELISA.Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC₅₀ of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.

Human

HEK293

C-hFc;C-His

Q86YL7 (M1-K123)

10630  [NCBI]

Approximately 50.2 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.